流动注射时间扫描荧光分析法测定青霉素V钾  

A time scanning fluorophotometric method for the determination of phenoxymenylpenicillin potassium by flow injection analysis

作　　者：高瑞[1] 马红燕[1] 

机构地区：[1]延安大学化学与化工学院,分析化学研究所,延安716000

出　　处：《分析试验室》2009年第11期10-13,共4页Chinese Journal of Analysis Laboratory

基　　金：陕西省教育厅专项科研基金(08JK489);延安大学研究生创新计划(yjc200817)项目资助

摘　　要：研究了H2SO4颜色反应用于青霉素V钾(PMPP)荧光测定的新方法。PMPP为弱荧光物质,与浓H2SO4反应后荧光显著增强。结合流动注射进样技术,借助时间扫描荧光方式,提出了流动注射时间扫描荧光分析法测定PMPP的新方法。在最大激发236.0 nm、最大发射波长306.0 nm处,PMPP在3.6×10-5g/L^4.0×10-2g/L范围内与荧光强度呈良好的线性关系,相关系数为0.9999。方法检出限为1.0×10-5g/L,相对标准偏差为0.6%(n=11,ρ=1.0×10-3g/L),进样量为0.18 mL。方法已用于药物及尿样中PMPP的测定。Phenoxymenylpenicillin potassium is a weak fluorescent substance and can react with sulfuric acid to produce stronger fluorescent species. Based on this, combined with flow injection analysis and time scanning technology,a new fluorimetric method for the detemfination of phenoxymenylpenieillin potassium by the color reaction of sulfuric acid is proposed. The experimental conditions that influence the fluorescence intensity were investigated and opti- mized. The excitation and emission wavelengths are 236.0 and 306.0 nm, respectively. The linear range is 3.6×10^-5g/L-4.0×10^-2g/L with the detection limit of 1.0×10^-5 g/L. The relative standard derivation is 0.6% （n = 11, ρ = 1.0×10^-3 g/L）, and the sampling amount is 0.18 mL. The method has been used for the determination of phenoxymenylpenicillin potassium in tablets and human urines with satisfactory results.

关 键 词：青霉素V钾 流动注射分析 时间扫描 荧光光度法 

分 类 号：O557.3[理学—热学与物质分子运动论]