6-氨基-5-氰基-3-甲基-4-(3-硝基苯)-1-苯基吡唑[3,4-b]并吡啶与人血清白蛋白结合作用的光谱特征  

Spectral characteristics of the interaction between 6-amino-5-cyano-3-methyl-4-(3-nitrophenyl)-1-phenylpyrazolo[3,4-b] pyridine(6A) and human serum albumin

作　　者：张轶[1] 冯素玲[1] 张新迎[1] 范学森[1] 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007

出　　处：《分析试验室》2009年第11期14-17,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20772025)项目资助

摘　　要：采用紫外光谱法和荧光光谱法研究了6-氨基-5-氰基-3-甲基-4-(3-硝基苯)-1-苯基吡唑[3,4-b]并吡啶(6A)与人血清白蛋白(HSA)的结合作用,利用同步荧光法和三维荧光法研究了6A与HSA作用前后人血清白蛋白的构象变化。观测到生理pH7.4条件下6A使HSA的紫外吸收峰增强,特征荧光峰猝灭。Stern-Volmer曲线显示,6A对HSA的荧光猝灭可能是一个单一的静态猝灭过程,并且得出18℃和37℃时的结合位点数和结合常数。根据F rster非辐射转移理论可求出6A与HSA作用距离r=3.73 nm;根据基本热力学参数ΔH、ΔS和ΔG判断6A和HSA主要通过氢键和范德华力发生相互作用。The binding interaction between 6-amino-5-cyano-3-methyl-4- （ 3-nitrophenyl）- 1- phenylpyrazolo [ 3,4-b ] pyridine（6A） and human serum albumin（HSA） was investigated by ultraviolet absorption and fluorseence spectra. Synchronous fluorescence and three-dimensional fluorescence spectra were used to study the structure change of HSA with the addition of 6A. In physiological condition （pH 7.4）, 6A led to the increasing of UV absorption and the quenching of intrinsic fluorescence of HSA. The Stem-Volrner curve shows that the quenching of 6A to HSA is probably a single static quenehing process. The binding sites number n and apparent binding constant K were measured at 18℃ and 37 qE. The distance （ r = 3.73 nm） between donor （HSA） and aeeeptor （6A） was obtained according to forster theory of non-radiation energy transfer. The thermodynamic parameters AH, AG and AS at different temperatures were calculated, respectively, which indicated that H-bond and Van der Waals played a major role in the interaction of 6A with HSA.

关 键 词：6-氨基-5-氰基-3-甲基-4-(3-硝基苯)-1-苯基吡唑[3 4-b] 人血清白蛋白 荧光光谱 同步荧光光谱 三维荧光光谱 紫外吸收光谱 

分 类 号：O557.3[理学—热学与物质分子运动论]