高效液相色谱-质谱联用法测定纺织品中的含氯苯酚  

Determination of the content of chlorinated phenols in textiles by liquid chromatography-tandem mass spectrometry

作　　者：洪爱华[1] 尹平河[1] 黄晓兰[2] 

机构地区：[1]暨南大学分析测试中心,广州510632 [2]中国广州分析测试中心,广州510070

出　　处：《分析试验室》2009年第11期88-90,共3页Chinese Journal of Analysis Laboratory

基　　金：广东省自然科学基金(07005971)项目资助

摘　　要：建立了高效液相色谱-质谱联用法检测纺织品中五氯苯酚（PCP）、四氯苯酚（TeCP）。选用ZORBAX XDB-C18柱（50 mm×2.1 mm,3.5μm）为分析柱,流动相为V（甲醇）∶V（纯水）=88∶12,流速为200μL/min;质谱条件选用气动辅助电喷雾离子源（ESI）,检测方式为负离子多离子反应检测（MRM）;PCP、TeCP标准曲线线性范围均为0.1-100μg/L;回收率为90%-99%;相对标准偏差为3.5%-7.6%（n=5）;方法检测低限为5μg/kg。方法适用于纺织品中PCP、TeCP的测定。A method was established for the determination of chlorinated phenols in textiles by liquid chromatography- mass spectrometry （HPLC-MS-MS）. ZORBAX XDB-C18 column （50mm × 2.1 mm, 3.5μm） was used with a mobile phase of methanol-water （88： 12）. Gas auxiliary electrospray ionization source （ESI） was used to detect the chlorinated phenols by the negative electrospray ionization and multiple reaction monitoring mode （MRM）. The calibration curves of tetrachlorophenol and pentachlorophenol were linear in the range of 0.1 to 100 ng/mL, and the hmit of quantitation was 5 μg/kg. The recovery was more than 90% and the RSD was less than 7.6%. The results show that this method established in the present research is accurate, sensitive, and specific. The method is suitable for simultaneous determination of chlorinated phenols in textiles.

关 键 词：高效液相色谱-质谱联用法 五氯苯酚 四氯苯酚 纺织品 

分 类 号：O557.63[理学—热学与物质分子运动论]