植物基因克隆技术的发展与展望  

Development and Prospect of the Gene Cloning Technology in Plant

作　　者：陈儒钢[1] 巩振辉[1] 逯明辉[1] 李大伟[1] 黄炜[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《长江蔬菜》2009年第10X期13-18,共6页Journal of Changjiang Vegetables

基　　金：国家自然科学基金(30571262);"十一五"国家科技支撑计划(2006BAD01A7);西北农林科技大学"青年学术骨干支持计划"(01140304);西北农林科技大学人才基金(01140508)

摘　　要：基因克隆技术是生命科学技术领域里非常重要的部分,为了纵览植物基因克隆的理论和技术的发展创新历程,对各种技术体系,正向遗传学途径、反向遗传学途径(包含定位克隆和同源序列克隆及随后发展起来的电子克隆)进行了综述。随着后基因组时代的到来,植物基因克隆技术将发挥更加重要的作用。Gene cloning technology is a very important in life science area. In order to insight the development and renovation process of gene cloning theories and techniques, many important cloning technique system applied in plant research were reviewed. Forward genetics strategy was based on expressed functions of genes, which by means of identifying the product or the mutant phenotype of gene. Forward genetics strategy included two aspects： functional cloning and phenotype cloning. Reverse genetics strategy was founded on the special location and sequences of gene itself, including positional cloning, homology cloning, and electronic cloning which developed in recent years. With the advent of post-genome era, plant gene cloning technology will play more important role in the future.

关 键 词：基因克隆 功能克隆 定位克隆 转座子标签法 抑制性差减杂交 同源序列法 基因芯片 电子克隆 

分 类 号：Q943.2[生物学—植物学]