补肾方对慢性乙型肝炎T淋巴细胞免疫效应分子表达的影响  

Effect of Bushen recipe on immune effector molecules of T lymphocytes

作　　者：李曼[1] 姚嫣[1] 高月求[1] 

机构地区：[1]上海中医药大学附属曙光医院肝肾疾病病证教育部重点实验室(上海中医药大学),上海200021

出　　处：《中西医结合肝病杂志》2009年第5期266-268,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：上海市教育委员会E研究院建设计划项目(No.03008);上海市科委引导项目(No.074119608);上海市浦东新区创新项目

摘　　要：目的:观察补肾方治疗肝肾阴虚兼湿热未尽型慢性乙型肝炎的免疫调控作用机制。方法:在曙光医院肝病门诊,收集HBV DNA阳性、ALT异常的肝肾阴虚型慢性乙型肝炎患者35例,随机分为治疗组和对照组,治疗组给予补肾方治疗,对照组给予α-2b干扰素治疗,疗程均为6个月,分别在用药前后用流式细胞法检测患者外周血T细胞的免疫效应分子穿孔素(perforin,PF)、颗粒酶B(granzyme B,GrB)、颗粒溶素(granulysin,GNLY)、TNF-α、IFN-γ的表达情况。结果:治疗组CD4+T细胞表达的GNLY、IFN-γ及CD8+T细胞表达的GrB、IFN-γ、TNF-α均明显降低。结论:补肾方通过减少T细胞免疫效应分子的表达,防止肝脏进一步受损,发挥治疗肝肾阴虚兼湿热未尽型慢性乙型肝炎的作用。Objective： To investigate the immunoregulation effect of Bushen rescipe （BSR） on chronic hepatitis B patients with liver-kidney Yin deficiency and lingering damp-heat. Methods： Thirty-five patients with HBV DNA positive and abnormal alanine transaminase （ALT） level were collected in Shuguang Hospital outpatient department, and they were randomized into treatment group and control group. The treatment group was treated with BSR for 6 months, and the control group was treated with α-2b interferon for 6 months. The expressions of immune effector molecules of T lymphocytes, including perforin, granzyme B （GrB）, granulysin （GNLY）, TNF-α and IFN-γ, were detected by flow cytometer. Results： The expressions of GNLY and IFN-γ of CIM_ T ceils and the expressions of GrB, TNF-α and IFN-γ of CD8 ＋ T ceils were declined in the treatment group. Conclusion： Bushen recipe can decline the expressions of immune effector molecules of peripheral T ceils in CHB patients with liver-kidney Yin deficiency, which contributes to the recovery of the damaged liver.

关 键 词：肝炎 乙型 慢性 补肾方/治疗应用 T淋巴细胞 穿孔素 颗粒酶B 颗粒溶素 肿瘤坏死因子-α 干扰素-γ 

分 类 号：R259[医药卫生—中西医结合]