亚甲蓝声化学法降解血液乙型肝炎病毒核酸的研究  

Study on inactivation of HBV DNA in blood by methylene blue sonochemical methods

作　　者：马小鹏[1] 章述军[2] 刘顺芳[1] 刘岩岩[1] 沈文状[1] 易继林[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院普外科,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院感染科,湖北武汉430030

出　　处：《中西医结合肝病杂志》2009年第5期283-284,共2页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:探讨利用超声化学的方法降解HBV DNA的可能性。方法:将HBV DNA阳性血清40例分为亚甲蓝超声化学组(MB-S组)、单纯超声组、单纯亚甲蓝组、对照组4组,每组各10例,分别予以相应的处理,应用荧光定量PCR技术观察HBV DNA降解情况。结果:MB-S组HBV DNA含量下降明显,与其他组比较差异有显著性意义。结论:MB-S法可以降解HBV DNA,这一新技术有重要的应用前景,值得深入研究。Objective： To observe the ability of inactivating HBV DNA in blood by methylene blue sonochemical methods. Methods： The samples of HBV DNA positive blood were divided into 4 groups randomly, methylene blue sonochemical group （MB-S group）, sonochemical alone group, methylene blue alone group and the control group. After the treatment as ac- cordingly, test the HBV DNA by RT-PCR. Results ： The efficiency of inactivation of HBV DNA in blood by MB-S group was significantly higher than the others （ P 〈 0.05 ） . Conclusion ： MB-S methods can inactivate HBV DNA in blood effectively, it may be a promising noveral method for virus inactivation.

关 键 词：肝炎病毒 乙型 亚甲蓝 声动力治疗 

分 类 号：R512.62[医药卫生—内科学]