玉米和苜蓿青贮发酵体系中菌群多样性的PCR-DGGE分析  

Analysis of monitor bacterial diversity in fermentive corn silage and alfalfa silage by PCR-DGGE method

作　　者：韩吉雨[1] 杨凯[2] 侯先志[1] 郭天龙[1] 赵子夫[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,呼和浩特010018 [2]北京农学院农业应用新技术北京重点实验室,北京102206

出　　处：《安徽农业大学学报》2009年第4期526-532,共7页Journal of Anhui Agricultural University

基　　金：内蒙古科技厅草业虚拟研究院(20040601)资助

摘　　要：利用PCR-DGGE研究玉米和苜蓿青贮在动态发酵过程中菌群的变化。结果表明,苜蓿青贮在青贮过程中菌群组成比较稳定,DGGE图谱上没有出现明显的条带增加或减少,青贮不同时间DGGE谱带相似性超过了79%;而玉米青贮在发酵3 d以后细菌种类和数量明显减少,0-3 d和7-60 d谱带的相似性不足50%;自然青贮（无论是玉米青贮还是苜蓿青贮）后期的优势菌群来源于未青贮前原料表面的原始菌群。在后续试验中对19条条带进行回收、测序,其中15条克隆成功,包括？-Lactococcus、？-Acinetob-acter、？-Streptococcus、？-Weissella、？-Pantoea、？-Escherichia、？-Enterobacter和？-Endophytic bacterium等几大类群。Changes in the composition of bacterial community in the corn silage and the alfalfa silage during the whole fermentation process were compared by using PCR-DGGE method.The results showed that the bacterial community in the alfalfa silage remained stable during the whole process.No clear bands disappeared or appeared on DGGE gel.The similarity indexes of the DGGE profiles during the whole process was over 79%.However,the bacterial community in the corn silage became clearly simple after 3 days fermentation.The similarity indexes of the DGGE profiles from 0 to 3 days and those from 7 to 60 days were less than 50%. The predominant bacteria of natural silage were originated from normal bacterial community of the original materials. In the subsequent experiment, 19 bands of agarose gel which contained target sequences were recycled, and 15 bands were successfully cloned,in- eluding ？-Lactococcus, ？-Acinetobacter, ？-Streptococcus, ？-Weissella, ？-Pantoea, ？-Escherichia, ？-Enterobacter, ？-Endophytic bacterium and so on.

关 键 词：玉米青贮 苜蓿青贮 PCR-DGGE 

分 类 号：S816.3[农业科学—饲料科学]