牛精子蛋白质组的双向电泳和质谱鉴定初步研究  

Primary studies on bovine sperm proteome by two-dimensional gel electrophoresis and mass spectrometry

作　　者：韩伟东[1,2] 王栋[2] 郝海生[2] 杜卫华[2] 赵学明[2] 朱化彬[2] 

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《安徽农业大学学报》2009年第4期538-542,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金项目(30671502);国家"863"高技术研究发展规划(2006AA10Z143);"跨越"计划项目"优质高产奶牛快速高效繁育技术集成与产业示范"和"现代农业产业技术体系(奶牛)"(nycytx-10)共同资助

摘　　要：通过建立牛精子双向电泳-质谱鉴定研究平台,为从蛋白质水平研究精子生理机能奠定技术基础;采用改进的热TRIzol法裂解精子细胞制备蛋白,应用双向凝胶电泳(2-DE)分离牛精子蛋白,通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)鉴定蛋白质点。得到了重复性较好的牛精子2-DE图谱,质谱鉴定16个蛋白质点。建立牛精子双向电泳-质谱鉴定研究平台,为后续牛精子X、Y差异蛋白点的检测和分析奠定了理论和技术基础。In order to lay a technological foundation for studying physiological function of spermatozoa,proteomics research of bovine spermatozoa with two-dimensional gel electrophesis and MALDI-TOF-MS were established.The heat TRIzol method and two-dimensional gel electrophoresis（2-DE） were used to separate the total proteins of bovine X and Y spermatozoa,ImageMaster 6.0 software was applied to analyze 2-DE images,and proteins were identified by both MALDI-TOF-MS and 2-DE.As a result,16 proteins in the spermatozoa were identified. So it provided a basis for detecting and analyzing the differential proteins of the X and Y bovine spermatozoa.

关 键 词：精子 蛋白质组学 双向电泳 基质辅助激光解吸电离飞行时间质谱 

分 类 号：S823.3[农业科学—畜牧学]