海带有性繁殖遗传机制的研究(英文)  

Genetic mechanism on sexual reproduction of Laminaria

作　　者：李玉晖[1,2] 王秀良[1] 段德麟[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071 [2]合肥学院生物与环境工程系,合肥230022

出　　处：《安徽农业大学学报》2009年第4期552-556,共5页Journal of Anhui Agricultural University

基　　金：国家自然基金项目(40376049,30500383);合肥学院引进人才基金项目(RC024)共同资助

摘　　要：以日本长海带(Laminaria longissima)×真海带(Laminaria japonica)杂交F1代60株幼孢子体为材料,利用RAPD、ISSR和AFLP 3种分子标记技术进行海带分子遗传机制的研究。结果表明:由25个RAPD引物、14个ISSR引物和20对AFLP引物组合共扩增出151个RAPD位点、89个ISSR位点和532个AFLP位点。其中符合尔孟德遗传分离规律的RAPD、ISSR和AFLP位点分别有125、87和430个,各占每种标记总扩增位点数的83.3%、97.8%和80.8%;偏离孟德尔遗传分离规律的RAPD、ISSR和AFLP位点分别有25、2和102个,各占每种标记总扩增位点数的16.7%、2.2%和19.2%。上述统计结果从分子水平上验证了海带有性繁殖过程符合孟德尔遗传方式。To investigate the genetic mechanism during the Laminaria sexual reproduction, the gametophytes of L. japonica Aresch and L. longissima Miyabe were chosen as parents for hybridization. RAPD, ISSR and AFLP makers were used for the analysis of genetic mechanism. All the 151 RAPD loci were amplified with 25 random primers ,89 ISSR loci were amplified with 14 SSR primers ,and 532 AFLP loci were amplified with 20 selective primer combinations. Among the amplified loci, 125 RAPD loci, 87 ISSR loci and 430 AFLP loci were fit for Mendel＇ s laws of inheritance and segregation among the F1 progenies, which accounted for 83.3%, 97.8% and 80.8%, respectively. It showed that the sexual reproduction of Laminaria basically followed Mendel＇s laws of inheritance and segregation.

关 键 词：海带 遗传机制 AFLP标记 ISSR标记 RAPD标记 

分 类 号：S968.421[农业科学—水产养殖]