一种大环柄菇属真菌的分子鉴定及其固体培养条件优化  

Molecular identification of Macrolepiota sp. H-603 and optimization of solid culture

作　　者：冯飞[1] 侯成林[1] 覃道春[2] 

机构地区：[1]首都师范大学生命科学学院,北京100048 [2]国际竹藤网络中心国家林业局竹藤科学与技术重点实验室,北京100102

出　　处：《安徽农业大学学报》2009年第4期573-579,共7页Journal of Anhui Agricultural University

基　　金：国家"十一五"科技支撑项目(2006BAD19B08;2006BAD19B04);国家林业局"948"项目(2006-4-71)共同资助

摘　　要：测定大环柄菇属一种真菌(Macrolepiotasp.)的rDNA ITS序列,根据序列构建系统发育树;采用固体培养方法在不同培养基和不同培养条件下对该菌菌丝体的生长条件进行分析,最后采用正交试验,考察碳源、氮源、pH值和矿质元素对该菌菌丝生长的影响。结果表明,Macrolepiotasp.与来自日本的Macrolepiota sp.Oku X-2000和Macrolepiota sp.TMI-23928亲缘关系近;正交试验结果表明,碳源、pH值、矿质元素和氮源对该菌菌丝体的生长速度有显著影响,固体培养条件优化结果为葡萄糖、豆饼粉、pH5且培养基中缺乏KCl。经优化后,菌丝体的生长速度可以达到2.28 mm.d-1。In this paper, the ITS region of nuclear ribosomal DNA of Macrolepiota sp. was sequenced, and a phylogenetic tree was set up based on ITS data. The solid culture method was selected to study the growth state of Macrolepiota sp. in different media and culture conditions. The results showed that the phylogenetic relationships of Macrolepiota sp. were closely related to Macrolepiota sp. Oku X-2000 and Macrolepiota sp. TMI-23928. The results of orthogonal experiment showed that C source, pH value, mineral element and N source had significant effect on the growth rate of the myeelia. The optimum solid culture conditions were glucose, bean cake powder, pH and lacking KC1 in the medium. The growth rate of mycelia was 2.28 mm· d^-1 under the optimized cultivation.

关 键 词：大环柄菇属 分子系统学 菌丝体 固体培养 条件优化 

分 类 号：Q949.32[生物学—植物学]