细脚拟青霉RCEF0788菌株抑制MAO活性产物的培养工艺研究  

Optimization of submerged culture of Paecilomyces tenuipes RCEF0788 for the highest MAO inhibitory activity

作　　者：王彪[1] 李春如[1] 宗文明[1] 樊美珍[1] 

机构地区：[1]安徽农业大学微生物防治省重点实验室,合肥230036

出　　处：《安徽农业大学学报》2009年第4期580-584,共5页Journal of Anhui Agricultural University

基　　金：国家高技术研究发展计划资助项目(863计划)(2007AA021506);国家自然科学基金项目(30570004);安徽省自然科学基金项目(070411011)共同资助

摘　　要：研究细脚拟青霉RCEF0788菌株抑制单胺氧化酶(MAO)活性产物的培养工艺。结果表明,综合MAO抑制率和发酵液提取物得率情况,较合适的氮源为酵母粉和蛋白胨;较合适的碳源为麦芽糖和乳糖;适宜的pH值为6.5。正交设计结果验证该菌株抑制MAO活性的最佳培养工艺为20 g.L-1酵母粉,20 g.L-1乳糖,10 g.L-1蛋白胨,10 g.L-1麦芽糖,1 g.L-1磷酸二氢钾和1 g.L-1硫酸镁,26.5℃,培养9 d。This paper is concerned with optimization of culture conditions for the highest MAO inhibitory activity production by Paecilomyces tenuipes RCEF0788.The results showed that yeast extract and peptone,maltose and lactose were suitable nitrogen,carbon,respectively,and the initial pH6.5 was suitable.The results of orthogonal experiment showed that the optimal concentration and culture conditions for the highest MAO inhibitory activity production by RCFE0788 were 20 g·L-1 yeast extract,20 g·L-1 lactose,10 g·L^-1 peptone, 10g·L^-1 maltose, 1 g·L^-1 KH2PO4 and 1 g·L^-1 MgSO4 ,26.5℃ for 9 d.

关 键 词：细脚拟青霉 单胺氧化酶抑制剂 正交试验 

分 类 号：Q949.32[生物学—植物学]