HPLC法测定阿图什市无花果叶中补骨脂素及佛手苷内酯的含量  

Determination of Psoralen and Bergaptenin Leaves of Ficus carica L. in Atushi by HPLC

作　　者：李军[1,2] 苏凡[2] 陈吉平[2] 孙保亚[3] 于黎明 顾连弟[4] 

机构地区：[1]大连市药品检验所,大连116021 [2]中国科学院大连化学物理研究所,大连116023 [3]沈阳市农业检测中心,沈阳110034 [4]辽宁中医药大学药学院,大连116600

出　　处：《中国医药导刊》2009年第9期1607-1609,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:建立无花果叶中补骨脂素和佛手柑内酯的含量测定方法。方法:采用反相高效液相色谱法测定补骨脂素和佛手柑内酯的含量,色谱柱kromasil C18 ODS(150mm×4.6mm,5μm),流动相为甲醇-水(65:35),测定波长为222nm,流速1mL·min^(-1),进样量4μl。结果:补骨脂素与佛手柑内酯线性良好,回归方程分别为:补骨脂素y=52850644.3891x+41975.0624,t=0.9991,线性范围:(0.01639～0.1639)μg;佛手柑内醇y=6958902012703x+31347.1000,r=0.9994,线性范围:(0.00296～0.0296)μg。平均回收率,补骨脂素为928%,RSD 1.25%;佛手柑内酯为95.7%,RSD 4.75%。结论:本法简便、准确、稳定,重复性好,可用于测定无花果叶中补骨脂素和佛手柑内酯的含量。Objective： To establish HPLC method for determination of psoralen and bergapten in leaves ofFicus carica L.Methods： RP- HPLC method was used to determine the content of psoralen and bergapten. Column kromasil C18 （150mm×4.6mm, 5μm） was used, with the flow rate of 1mL.min^-1, the mobile phase of methanol-water （65：35） and the detection wavelength of 222 nm.Results： There was a good linear relationship over the concentration range of （0.01639-0. 1639）μg with r of 0.9991 for psoralen and （0.00296-0.0296）μg with r of 0.9994 for bergapten. The average recoveries of psomlen and bergapten were respectively 97.8%, RSD 1.25% and 95.7%, RSD 4.75%.Conclusion： The method is simple, accurate and stable. It could be applied to the determination of quality control.

关 键 词：无花果叶 补骨脂素 佛手柑内酯 含量测定 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]