紫茎泽兰hsp90和hsp17.66基因启动子的克隆及其序列分析  

Cloning and Sequence Analysis of Promoters in hsp90 and hsp17.66 Genes from Ageratina adenophora

作　　者：宫伟娜[1,2] 万方浩[1] 谢丙炎[2] 郭建英 周艳[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100081 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《热带亚热带植物学报》2009年第5期451-457,共7页Journal of Tropical and Subtropical Botany

基　　金：国家973计划项目(2009CB119200)资助

摘　　要：以入侵植物紫茎泽兰(Ageratina adenophora Sprengel)为材料,采用基于PCR方法的染色体步行和改良的热不对称交错PCR(thermal asymmetric interlaced PCR,TAIL-PCR)两种方法分别克隆到hsp90和hsp17.66基因的5′上游启动子序列,长度分别为864 bp和1 485 bp(GenBank登录号分别为FJ434253和FJ434252)。测序结果表明:这两个启动子序列均具有hsp启动子特有的HSE元件,及其他一些启动子顺式作用元件,如TATA-box,CAAT-box等。The promoter region of hsp90 with 864 bp （GenBank No. FJ434253）, from invasive weed Ageratina adenophora was amplified based on PCR methods, which are available as chromosome walking from a known sequence to an unknown region. Moreover, another promoter region of hsp17.66 with 1 485 bp （GenBank No. FJ434252） was cloned by thermal asymmetric interlaced PCR （TAIL-PCR）. Both of the 5＇-flanking regions of hsp90 and hsp17.66 contained specific HSE element and several cis-acting elements, such as TATA-box, CAAT-box,et al.

关 键 词：紫茎泽兰 热激蛋白基因 启动子 克隆 染色体步行 TAIL-PCR 

分 类 号：S451[农业科学—植物保护] Q943.2[生物学—植物学]