硝酸亚铈对小鼠睾丸细胞能量代谢相关酶活性、细胞周期及凋亡的影响  

Effect of cerium nitrate on activity of the enzyme related to energy metabolism,cell cycle and apoptosis of testiculus cell in male mice

作　　者：赵海军[1] 王宝贵[2] 张桂英[2] 张惠[2] 周静[3] 凌丰[1] 陈敏华[1] 

机构地区：[1]浙江医学高等专科学校公共卫生系,浙江杭州310053 [2]吉林大学公共卫生学院,吉林长春130021 [3]杭州中肽生化有限公司,浙江杭州310018

出　　处：《西安交通大学学报（医学版）》2009年第5期559-561,591,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：浙江医学高等专科学校科研基金资助项目(2008B02);浙江省大学生科技创新项目(2008)

摘　　要：目的探讨硝酸亚铈对于小鼠睾丸细胞能量代谢相关酶类活性、细胞周期以及凋亡的影响。方法100只雄性小鼠随机分为阴性对照组、环磷酰胺组及硝酸亚铈15、30、60 mg/kg组,各组动物用相应受试物连续灌胃60 d。采用酶化学方法检测乳酸脱氢酶(LDH)、山梨醇脱氢酶(SODH)、琥珀酸脱氢酶(SDH)、葡萄糖-6磷酸脱氢酶(G-6PD)的活性;采用流式细胞仪检测睾丸细胞的细胞周期及细胞凋亡。结果与环磷酰胺组比较,15 mg/kg硝酸亚铈能促进LDH、SODH、SDH及G-6PD活性(P<0.05),30 mg/kg硝酸亚铈有促进SDH活性的作用(P<0.05);15 mg/kg和30 mg/kg硝酸亚铈均能延长睾丸细胞G0/G1期,缩短S期和G2+M期,抑制细胞凋亡(P<0.05)。与阴性对照组比较,60 mg/kg硝酸亚铈对LDH、SDH、G-6PD均有显著性抑制作用(P<0.05),且能缩短睾丸细胞G0/G1期,延长S期和G2+M期,细胞凋亡明显增加(P<0.05)。结论低剂量的硝酸亚铈对小鼠睾丸细胞能量代谢相关酶活性和细胞增殖有促进作用,而高剂量硝酸亚铈则表现出明显的抑制作用。Objective To explore the effect of cerium nitrate on activity of the enzyme related to energy metabolism, cell cycle and apoptosis of testiculus cell in male mice. Methods A total of 100 male mice were distributed randomly into negative control group, cytoxan group, cerium nitrate groups in low dose （15 mg/kg）, medium dose （30 mg/kg）, high dose （60 mg/kg）. Whole mice were given corresponding reagent for 60 days. Enzyme-chemical method was used to detect the activity of lactate dehydrogenase （LDH）, sorbitol dehydrogenase （SODH）, succinic dehydrogenase （SDH）, glucose-6-phosphate dehydrogenase （G-6PD）. Cell cycle and apoptosis of testiculus cell were studied by the way of one-fluorescence dying method on flow cytometer. Results 15 mg/kg cerium nitrate could promote the activity of LDH, SODH, SDH and G-6PD of testiculus cell compared with that of cytoxan （P^0.05）. 30 mg/kg cerium nitrate could advance the activity of SDH of. testiculus cell with cytoxan （P〈 0.05）. 15 mg/kg and 30 mg/kg cerium nitrate could extend Go/G1 and shorten S and G2 ＋M of cell cycle, inhibit apoptosis compared with cytoxan （P〈0.05）. 60 mg/kg cerium nitrate could inhibit the activity of LDH, SDH and G-6PD of testiculus cell with negative control group （P 〈 0.05）, extend S and G2 ＋M and shorten G0/G1 of cell cycle, and increase apoptosis compared with negative control group （P〈 0.05）. Conclusion Low-dose cerium nitrate promotes activity of enzyme related energy metabolism and proliferation of testiculus cell, while high-dose cerium nitrate shows adverse effects.

关 键 词：硝酸亚铈 细胞周期 凋亡 生殖毒性 

分 类 号：R994.6[医药卫生—毒理学]