甲基苯丙胺对映异构体两种衍生化方法的比较分析  

Comparison of two derivatization methods for enantiomer profiling of methamphetamine

作　　者：张建新[1] 张大明[1] 韩旭光 乔静[1] 杨士云[1] 辛国斌[1] 

机构地区：[1]北京市公安局法医检验鉴定中心,北京100192 [2]公安部缉毒局,北京100741

出　　处：《中国法医学杂志》2009年第5期289-292,共4页Chinese Journal of Forensic Medicine

基　　金：国家科技部十一五攻关资助项目(2006BAK09B08)

摘　　要：目的建立甲基苯丙胺毒品的对映异构体分析的优化方法。方法按文献方法用(S)-(+)-a-甲氧基-a-(三氟甲基)苯基乙酰氯(MTPACl)对甲基苯丙胺直接衍生化和在碱性条件下用有机溶剂萃取后再用MTPACl衍生化(对文献方法优化),分别对其衍生物采用全扫描形式进行GC-MS分析,比较其结果。结果优化方法的检测限低,峰型好,副反应少。文献方法的检测限是0.1ng,优化方法的检测限是0.001 6ng。结论优化方法用于甲基苯丙胺的对映体特征分析,结果更准确,实用性更强。Objective A procedure to analyze enantiomer profiling of methamphetamine was optimized. Methods According to the procedure optimized by us, methamphetamine samples were extracted by organic solvent from alkali aqueous solution and then derivatized with （S）-（ ＋ ）-MTPAC1）. To another procedure introduced in a literature, methamphetamine isomers were directly derivatized with （S）-（ ＋ ）-a- methoxy-a-（trifluoromethyl）phenylacetyl chloride （（S）-（ ＋ ）-MTPAC1）. The both derivatives were ana- lyzed by GC-MS in full scan monitoring mode. Result The optimized procedure have better limit of detection, better peak and few side reaction. The limit of detection was 0.1 ng in the literature. The limit of detection using optimized method was 0. 001 6ng. Conclusion The optimized procedure could be used to analyze enantiomer profiling of methamphetamine practically.

关 键 词：法医毒物分析 甲基苯丙胺 GC-MS 对映异构体 

分 类 号：D919[医药卫生—法医学]