死亡大鼠组织中总RNA提取的质量控制及方法评价  

Quality control and methods evaluation on extraction of total RNA from dead rats tissues

作　　者：任广睦[1] 潘一民[2] 刘季[3] 孙俊红[1] 王英元[1] 

机构地区：[1]山西医科大学法医学院,山西太原030001 [2]太原市公安局,山西太原030001 [3]广州市公安局交警大队,广东广州510640

出　　处：《中国法医学杂志》2009年第5期318-320,共3页Chinese Journal of Forensic Medicine

基　　金：山西省高校科技研究开发项目(20051224);山西医科大学博士启动基金(03200716)

摘　　要：目的探讨不同商品化试剂盒在提取组织总RNA过程中的优劣与适用范围,为实验室的质量控制提供依据。方法采用以膜提取技术为基础(SV total RNA isolation system)和以异硫氰酸胍/苯酚法为原理(TRIzol总RNA提取试剂盒)的两种试剂盒对死亡大鼠心、肝、脾、肺、肾、脑组织进行总RNA的提取与评价鉴定,并使用琼脂糖变性凝胶电泳和2100芯片生物分析仪对提取的总RNA进行完整性鉴定和质量控制评价。结果两种商品化试剂盒提取的总RNA纯度与得率均可满足实验要求。各组织脏器中总RNA得率依次为脾脏>肝脏>肾脏>大脑>肺脏>心脏。结论与TRIzol总RNA提取试剂盒相比,SV Total RNA Isolation System提取时对操作人员熟练度要求较低,2100芯片生物分析仪有望替代凝胶电泳技术用于总RNA提取时的质量控制。Objective To evaluate the two different commercial reagents in extraction of total RNA in forensic experiments. Methods Total RNA was extracted from heart, liver, spleen, lung, kidney and brain samples of dead rats using TRIzol reagent and SV Total RNA Isolation System according to the manufacturers＇ instructions. The purity, concentration and yield of total RNA were measured using gel electrophoresis and 2100 chip bio-analysis instrument. Results The two different commercial reagents both can satisfy the basic requirements of the experiments. The highest RNA yield was found in spleen and the lowest in heart. Conclusion Compared with the TRIzol reagent, SV Total RNA Isolation System is better for forensic experiments. The 2100 chip bio-analysis method is expected to be used in the quality control of total RNA extraction.

关 键 词：法医病理学 质量控制 总RNA提取 SV总RNA纯化系统 

分 类 号：D919[医药卫生—法医学]