高迁移率族蛋白B1在白细胞介素-2转录表达信号调控中的作用  

High mobility group box-1 protein plays an important role in transcription and expression of interleukin-2 mediated by nuclear factor of activated T cells-2

作　　者：刘辉[1] 姚咏明[2] 宋青[1] 丁丽华[3] 王晓辉[3] 袁斌[3] 叶棋浓[3] 盛志勇[2] 

机构地区：[1]解放军总医院外科重症医学科,北京100853 [2]解放军总医院第一附属医院烧伤研究所 [3]军事医学科学院生物工程研究所

出　　处：《中华急诊医学杂志》2009年第10期1069-1073,共5页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金（30801187,30672178）;国家重点基础研究发展规划项目（2005CB522602）

摘　　要：目的探讨高迁移率族蛋白B1（HMGB1）在白细胞介素-2（IL2）转录表达信号调控机制中的可能作用。方法首先将HMGB1和NFAT2质粒共同转染Hela细胞，同时转染IL-2报告基因，逐步增加HMGB1的转染剂量，检测IL-2报告基因的表达活性。观察HMGB1质粒用量对IL-2报告基因活性的影响；然后应用SRNAi质粒对内源性及外源性HMGB1表达进行特异性抑制，观察对IL-2报告基因活性的影响，以期从反面论证HMGB1可促进IL-2转录表达。结果在Hela细胞中，随着HMGB1质粒转染剂量增加，IL-2报告基因活性增加了2．12倍（P〈0．01）。应用sRNAi抑制293T细胞中外源性以及Hela细胞中内源性HMGB1表达水平后，IL-2报告基因活性分别下降1．7倍和4．76倍（P〈0．05或P〈0．01）。结论HMGB1在NFAT2介导IL-2报告基因转录表达的信号调控过程中发挥重要作用。Obejective To investigate the potential role of high mobility group box-1 protein （HMGB1） in transcription and expression of interleukin （IL）-2 mediated by nuclear factor of activating T cells 2 （NFAT2）. Method Firstly, HMGB1 and NFAT2 plasmids were transfected into Hela cells. Doses of HMGBI plasmid increased from 0 to 0.4μg/plate steadily and reporter gene activity of IL-2 was detected and compared. Secondly, endogenous HMGB1 in Hela cells or exogenous HMGB1 in 293T cells was inhibited respectively by sRNAi. Doses of sRNAi for HMGB1 was arisen from 0 to 1.2 μg/plate steadily and reporter gene activity of IL-2 was detected and compared. Results Reporter gene activity of IL-2 increased by 2.12-fold induced by increasing transfection-dose of HMGB1 plasmid in Hela cells （ P 〈 0.01）. However, reporter gene activity of IL-2 decreased by 1.7-fold and 4.76-fold repectively due to specific inhibition of exogenous HMGB1 in 293T cells and endogenous HMGB1 in Hela cells by increasing dose of sRNAi plasmid （ P 〈 0.05 or P 〈 0.01 ）. Conclusions HMGB1 plays an important role in transcription and expression of IL-2 mediated by NFAT2.

关 键 词：高迁移率族蛋白B1 活化T细胞核因了 白细胞介素2 细胞因子 小RNA干扰 信号转导 基因表达 免疫 

分 类 号：R459.7[医药卫生—急诊医学]