MDM2、P53和P27蛋白在食管鳞癌及其癌旁组织中的表达及其意义  

Expressions and Significances of MDM2,P53 and P27 Proteins in Esophageal Carcinoma and Adjacent Tissues

作　　者：李超霞[1] 方力[1] 杨小红[1] 

机构地区：[1]暨南大学医学院第四附属医院广州市创伤外科研究所,广东广州510220

出　　处：《癌变．畸变．突变》2009年第5期362-366,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:探讨癌基因蛋白MDM2、抑癌基因蛋白P53以及细胞周期蛋白P27在食管鳞癌及其癌旁组织中的表达及其意义。材料与方法:采用免疫组织化学EnVision二步法(定性)检测85例食管癌存档蜡块及其癌旁黏膜中MDM2、P53和P27蛋白的表达;采用流式细胞仪(定量)检测上述3种蛋白在48例食管癌新鲜组织标本及其癌旁黏膜以及12例切缘相对正常黏膜中的表达。结果:从单纯性增生-轻度非典型增生-中度非典型增生-重度非典型增生-原位癌-浸润癌进展过程的变化,发现:定性和定量检测结果均显示P53蛋白在正常食管黏膜上皮中无表达,在食管癌变早期即出现P53蛋白的积聚;而MDM2、P27蛋白在正常黏膜上皮均有不同程度的表达,在癌变的晚期MDM2蛋白表达明显增加。结论:P53和P27蛋白表达的变化可能发生在食管癌形成早期,MDM2蛋白表达的变化可能发生在食管癌变的晚期。定性和定量2种方法联合检测能更客观和准确的探讨癌基因产物的表达及其临床意义。BACKGROUND AND AIM：To examine the expressions and their significance of oncogene protein MDM2,anti-oncogene protein P53 and cell cycle protein P27 in esophageal squamous cell carcinom and adjacent mucosa.MATERIALS AND METHODS：Immunohistochemistry was used to detect the expressions of MDM2,P53 and P27 proteins in eighty-five cases of esophageal squamous cell carcinoma and their corresponding adjacent mucosa.Flow cytometry（FCM） was applied to measure the quantities of these protein expressions in 48 fresh esophageal squamous cell cancers and adjacent tissue and 12 relatively normal mucosa at the edge of the excision. RESULTS： The changes of esophageal epithelia from simple hyperplasia to mild to moderate to severe dysplasia to carcinoma in situ and infiltrating carcinoma, revealed that the P53 protein was not expressed in normal mucosa but appeared in the early stages of esophagus carcinogenesis. The MDM2 and P27 proteins had different degrees of expression in normal esophageal mucosa, with MDM2 protein markedly increased in the advanced stages of esophageal cancer. CONCLUSION： P53 and P27 proteins appeared in the early stages of esophagus oncogenesis, however the changes of MDM2 expression occurred in the advanced stage of esophageal could expl carcinogenesis. Flow cytometry quantitative ore the expressions of oncoproteins and their and immunohistochemistry qualitative analyses of gene proteins, clinical significances.

关 键 词：食管鳞癌 癌基因蛋白 免疫组化 流式细胞术 

分 类 号：Q786[生物学—分子生物学]