运动对胰岛素抵抗大鼠血清视黄醇结合蛋白4及骨骼肌磷脂酰肌醇3激酶表达的影响  

Effects of exercises on expressions of retinol binding protein-4 in serum and phosphatidylinositol 3 kinase in skeletal muscles of insulin resistant rats

作　　者：张黎军[1] 刘慧红[1] 黄从新[2] 

机构地区：[1]武汉大学人民医院老年病科,武汉430060 [2]武汉大学人民医院心血管内科,武汉430060

出　　处：《中华物理医学与康复杂志》2009年第10期659-662,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的研究运动对胰岛素抵抗（IR）大鼠血清视黄醇结合蛋白4（RBP4）及骨骼肌磷脂酰肌醇3激酶（P13K）表达的影响。方法将30只雄性Wistar大鼠随机分为对照组、IR组及运动组，后2组大鼠均给予高糖高脂饲料喂养8周，经证实IR造模成功后，IR组及运动组大鼠继续给予高糖高脂饲料喂养6周，运动组同时进行为期6周的游泳训练。于实验进行14周末时检测各组大鼠体重（BW），然后处死大鼠取血检测血糖（FPG）、血清胰岛素（FINS）、甘油j酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL．C）及低密度脂蛋白胆同醇（LDL—C）水平，并计算胰岛素抵抗指数（HOMA—IR），选用ELISA技术检测血清RBP4水平，采用免疫组化法检测大鼠骨骼肌巾P13K表达；另外提取各组大鼠肠系膜、附睾、反折腹膜脂肪等组织称重，计算内脏脂肪重量与体重的比值。结果（1）运动组大鼠血清RBP4、TC、TG、FPG水平均显著低于IR组；IR组大鼠内脏脂肪重量、内脏脂肪重量／体重比值、血清RBP4、FINS、LDL、HOMA—IR均高于其它两组（P〈0．01）；血清HDL水平均低于其它两组（P〈0．01）。（2）运动组大鼠骨骼肌P13K表达强度明显高于IR组（P〈0．01）。（3）通过相关性分析发现，IR大鼠血清RBPd与FINS、内脏脂肪重量／体重比值、LDL、HOMA—IR具有正相关性；与HDL、骨骼肌P13K水平具有负相关性。结论运动能下调IR大鼠血清RBP4水平及增强骨骼肌P13K表达，对改善机体胰岛素敏感性具有重要意义。Objective To investigate the effects of exercises on expressions of retinol binding protein-4 （RBP4） in serum and pbosphatidylinositol 3 kinase （P13K） in skeletal muscles of insulin-resistant （IR） rats in- duced by diets. Methods A total of 30 Wistar rats were randomly divided into 3 groups： normal control group fed with normal diet, IR and exercises groups with high sugar and high fat diet. After 8 weeks, the Wistar rats in exercises group took swimming training for 6 weeks and all groups kept their assigned diets. At the end of 14-week experiment, the body weight （BW） of rats were measured, the general behaviors of rats were observed and then sacrificed. Rats＇ blood were sampled for measuring the levels of glucose （FPG） and serum RBP4, insulin （FINS） , HDL-C, LDL-C, TG, TC. The indexes of [R （HOMA-IR） were calculated. The levels of RBP4 in serum were measured with ELISA technique. The ratios of visceral fat content to BW were calculated too. Immunohistochemis- try method was applied to detect the expression levels of PI3K in skeletal muscle. Results （ 1 ） The levels of RBP4, TC, TG, FPG in serum in exercises group were lower than those in IR group significantly; the visceral fat content, ratio of visceral fat content to BW, FINS, RBP4, LDL and HOMA-IR in serum increased significantly in IR group after 6 weeks feeding compared with normal control group and exercises group （P 〈 0.01 ） ; the levels of HDL in serum in IR group were lower than those in the other two groups. （2） The expression levels of PI3K in skeletal muscles in exercises group were significantly higher than that in IR group （ P 〈 0.01 ）. （ 3 ） in a muhiple stepwisc regression analysis, FINS, ratio of visceral fat content to BW, LDL and HOMA-IR were correlated with RBP4 positively; HDL and PI3K were correlated with RBP4 negatively. Conclusion Exercises could downregu- late the level of RBP4 in serum and upregulate the level of PI3 K in skeletal muscles of IR rats. This effect was im- portant f

关 键 词：运动 视黄醇结合蛋白4 磷脂酰肌醇3激酶 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]