离体乳内动脉乙酰胆碱M受体亚型的功能研究  

Study on the Function of Muscarinic Cholinergic Receptor Subtypes of Internal Mammary Artery in Vitro

作　　者：吕航[1,2] 迟超[1] 刘宗泓[1] 张学峰[1] 王柏春[1] 张国伟[1] 刘宏宇[1] 

机构地区：[1]哈尔滨医科大学附属第一医院心血管外科,哈尔滨150001 [2]哈尔滨医科大学附属第二医院心外科,哈尔滨150001

出　　处：《中国胸心血管外科临床杂志》2009年第5期374-379,共6页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：黑龙江省自然科学基金资助项目(D2004-24);黑龙江省卫生厅科研基金资助项目(2003-067)~~

摘　　要：目的为了从胆碱能受体角度研究抑制围术期乳内动脉(IMA)痉挛的方法,对离体乳内动脉胆碱能受体亚型的功能进行研究。方法取30例行冠状动脉旁路移植术(CABG)患者内皮完整的离体乳内动脉血管段,根据应用不同浓度的拮抗剂处理,采用随机数字表法分为对照组(未用拮抗剂)、阿托品组(非选择性M受体拮抗剂)、哌仑西平组(M1受体拮抗剂)和美索曲明组(M2受体拮抗剂)。分析不同浓度拮抗剂对血管舒张反应的影响,应用Scott比值法计算受体亲和力指数(pD2),应用Schild plot作图法求出拮抗指数(pA2)。结果乙酰胆碱介导氯化钾预收缩的内皮完整的乳内动脉是浓度依赖的舒张反应,其对乙酰胆碱的pD2为6.92±0.05。阿托品组、哌仑西平组、美索曲明组对内皮完整的乙酰胆碱介导的剂量-效应曲线的影响均是浓度依赖性的,随着拮抗剂浓度的增加,乙酰胆碱介导的剂量-效应曲线均明显右移(P<0.05)。阿托品、哌仑西平、美索曲明竞争性拮抗血管对乙酰胆碱的反应,其pA2分别为9.62±0.15,7.70±0.08和6.30±0.08。结论乙酰胆碱介导的内皮完整的乳内动脉舒张反应是浓度依赖性的。根据不同拮抗剂的亲和力不同,离体乳内动脉乙酰胆碱介导的舒张反应是通过作用于非神经性乙酰胆碱M1受体亚型实现的。Objective To study the method to inhibit perioperative internal mammary artery （IMA） spasm from the perspective of muscarinic receptor, and research the function of muscarinic cholinergic receptor subtypes of IMA. Methods IMA segments in vitro with intact endotbelium were obtained from 30 patients who underwent coronary artery bypass grafting （CABG）. According to muscarinic receptor antagonists of different concentrations, They were divided into control group （not using receptor antagonist）, atropine group （non-selectlve M receptor antagonist）, pirenzepine group （M1 receptor antagonist） and Methoetramine group（M2 receptor antagonist） by random number table. The effects of antagonists on vasodilatation were analyzed, Scott ratio was used to calculate affinity index （pD2） and Sebild plot was used to count rivalry index （pA2）. Results Acetylcholine （Aeh）-induced concentration- dependent relaxation response of IMA segments with intact endothelium precontraeted with potassium chloride （KC1）. The pD2 was 6.92±0.05. The effects of atropine, pirenzepine and methoctramine on dose-response curve induced By Acb with intact endotbelium were all concentration dependent. With the increase of the concentration of antagonists, the Ach-induced dose response curves had a significant shift to right （P〈 0. 05）. Atropine, pirenzepine and Metboctramine competitively antagonized the reaction of vessel to Ach. The pA2 were 9. 62±0. 15,7. 70 ± 0.08 and 6.30± 0. 08, respectively. Conclusion The Ach-induced relaxation response of IMA with intact endotheliurn is concentration-dependent. According to the affinity of different antagonist, IMA in Vitro Ach induced relaxation response is implemented by acting on non-neuronal muscarinic cbolinergic M1 receptor subtype.

关 键 词：冠状动脉旁路移植术 非神经性乙酰胆碱能受体亚型 血管舒张 乳内动脉 

分 类 号：R654.2[医药卫生—外科学]