系统性红斑狼疮患者外周血单一核细胞CXCR3、CXCR5 mRNA的表达  

Expression of CXCR3 and CXCR5 mRNA in peripheral blood mononudear cells of the patients with systemic lupus erythematosus

作　　者：李遇梅[1] 姚煦[2] 高建明[3] 陈敏[2] 李安生[2] 杨桂兰[4] 陈志强[2] 

机构地区：[1]江苏大学附属医院皮肤科,镇江212001 [2]中国医学科学院皮肤病研究所,南京210042 [3]北京市海淀区医院皮肤科 [4]兰州军区总医院皮肤科

出　　处：《中国麻风皮肤病杂志》2009年第10期729-732,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金课题编号:30170863;江苏省自然科学基金编号:BK2001195;江苏省医学重点学科建设与人才战略"135工程"资助

摘　　要：目的:检测趋化因子受体CXCR3及CXCR5在系统性红斑狼疮(SLE)患者外周血单一核细胞(PBMC)中的表达,并分析其与疾病活动性的相关性。方法:收集93例SLE患者和30例正常对照者的外周血单一核细胞,提取RNA。应用RT-PCR方法检测CXCR3、CXCR5mRNA的表达。结果:活动期SLE CXCR3 mRNA的表达高于健康对照组。活动期SLE患者CXCR5 mRNA的表达均高与非活动期和健康对照组。SLE患者组CXCR5 mRNA表达水平与SLE疾病活动指数(SLEDAI)呈正相关。结论:CXCR3和CXCR5 mRNA的高表达提示SLE处于活动期。Objective： To detect the expression of chemokine receptors CXCR3 and CXCR5 in peripheral blood mononuclear cells （PBMC） of the patients with systemic lupus erythematosus （SLE） and to analyze the relationship between the expression and the activity of the disease. Methods： RNA was abstacted from PBMC and the expression of CXCR3 and CXCR5 was detected by RT- PCR in 93 SLE patients and 30 controls. Results： The level of CXCR3 expression was significantly increased in active patients than in controls. The level of CXCR5 expression was significandy higher in the active patients than in inactive patients and controls. The expression of CXCR5 was correlated with SLE disease activity index （SLEDAI）. Conclusion： The high expression of CXCR3 and CXCR5 may indicate the activity of SLE.

关 键 词：系统性红斑狼疮 趋化因子受体 CXCR3 CXCR5 

分 类 号：R593.241[医药卫生—内科学]