斑秃患者外周血单核细胞STAT4和血清IL-12及IFN-γ的表达  

Expression of IL-12, IFN-γ and STAT4 in patients with alopecia areata

作　　者：黄峥烨[1] 秦海红[2] 谢韶琼[1] 王海俊[1] 汪青良[1] 王秀丽[1] 徐伟民[1] 

机构地区：[1]上海市皮肤病性病医院,上海200050 [2]上海市第一人民医院宝山分院皮肤科,上海200940

出　　处：《中国麻风皮肤病杂志》2009年第10期739-741,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的:检测斑秃患者外周血单核细胞STAT4和血清IL-12及IFN-γ的表达。方法:应用RT-PCR方法检测50例斑秃患者和25名正常人外周血单核细胞(PBMC)中STAT4的mRNA表达;ELISA法检测IL-12和IFN-γ的表达。结果:活动期斑秃患者中IL-12、STAT4和IFN-γ的水平显著高于缓解期患者(均P<0.05)和正常人(均P<0.05);病程>3个月的患者IL-12、STAT4和IFN-γ的表达显著高于病程<3个月的患者,但二者均高于正常人。结论:斑秃患者中存在IL-12和STAT4的高表达,可能通过IL-12/STAT4/IFN-γ途径直接或间接诱导IFN-γ的过量表达,导致毛囊部位炎症形成,引起脱发。Objective： To detect the expression of IL- 12, STAT4 and IFN -γ. Methods： Real time PCR was applied to detect the expression of STAT4 mRNA in peripheral blood mononuclear cell （PBMC）, and ELISA was used to determine the level of IL - 12 and IFN -γ in sera from 50 patients with alopecia areata and 25 healthy controis. Results： The expression of IL - 12, STAT4 and IFN -γ was significantly higher in active alopeeia areata patients than in inactive alopeeia areata patients （ P 〈 0.05 ） and normal controls （ P 〈 0.05）. The expression of IL - 12, STAT4 and IFN - γ was significandy higher in the patients with a duration more than 3 months than the patients with a duration less than 3 months （ P 〈 0.05）. Conclusion： The high expression of IL- 12 and STAT4 in alopecia areata may induce inflammation of hair follicles and alopecia caused by over - production of IFN - γ, which is induced through a IL- 12/STAT4/IFN-γ/activation pathway directly or indirectly.

关 键 词：斑秃 IL-12 STAT4 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]