小鼠单纯疱疹病毒性脑炎主要免疫反应特性研究  

The Immune Responses Characteristics of Mouse Herpes Simplex Encephalitis

作　　者：张敏[1] 梅元武[2] 孙欣[3] 

机构地区：[1]华中科技大学同济医学院附属协和医院康复科,武汉430022 [2]华中科技大学同济医学院附属协和医院神经内科,武汉430022 [3]华中科技大学同济医学院附属协和医院放射科,武汉430022

出　　处：《卒中与神经疾病》2009年第5期313-316,共4页Stroke and Nervous Diseases

基　　金：湖北省科技攻关项目(2007AA301823-1)

摘　　要：目的了解单纯疱疹病毒性脑炎(HSE)小鼠的主要免疫反应特性。方法Balb/c小鼠颅内注射HSV1病毒制造HSE模型,脑组织切片观察病理变化,流式细胞仪检测CD11b、CD40、MHCⅠ、MHCⅡ抗原表达水平,RT-PCR检测脑内细胞因子(IL-2、IL-4、IL-10、TNF-α)mRNA表达水平。结果HSE小鼠脑组织片状坏死出血。HSE小鼠表达CD11b、CD40、MHC、MHC增加,TH1型细胞因子(IL-2、TNF-α)、TH2型细胞因子(IL-4、IL-10)显著增高。结论小胶质细胞感染后被激活、增殖,MHC增加以发挥抗原提呈作用;CD40表达将进一步激活小胶质细胞,促进TH1及TH2型细胞因子分泌。Objective To investigate the characteristics of the three courses of immune responses in herpes simplex encephalitis （HSE） of Batb/c mouse. Methods Balb/c mouse HSE model was made by injecting herpes simplex virus type I （HSV1） and their brain tissue was obtained. Pathologic slices of brain tissues of mice were used to identify their morphologic difference. The surface antigens on microglia, such as CD11b, CD40, MHC Ⅰ and MHC Ⅱ were measured by flow cytometry and semiquantitative reverse transcription polymerase chain reaction （RT-PCR） were used to detect the mRNA of IL-2, IL-4, IL-10, TNF-α and iNOS in mice＇s brains. The results of the experiment were operated by Student Test with SPSS 10. 0 software. Results After herpes simplex virus typel （HSV1） infection, the lesions of haemorrhage and necrosis in mice＇s brains were found by microscope, and the expression levels of surface antigen CD11b. CD40. MHC Ⅰ . MHC Ⅱ and mRNA of IL-2, IL-4, IL-10, TNF-α and iNOS increased. Conclusions Microglial cells play an important roles in HSE as antigen presenting cells： they are in activation and proliferation and they present surface antigens such as MHC and CD40 in favor of microglia presenting virus antigens and lymphocyte permeating blood-brain barrier and microglia reactivating, secreting cytokines. TH1 type cytokines and TH2 type cytokines both play important roles in HSE.

关 键 词：单纯疱疹病毒性脑炎 免疫应答 小胶质细胞 

分 类 号：R512.3[医药卫生—内科学]