Loosening Xyloglucan Accelerates the Enzymatic Degradation of Cellulose in Wood  

作　　者：Rumi Kaida Tomomi Kaku Kei'ichi Baba Masafumi Oyadomari Takashi Watanabe Koji Nishida Toshiji Kanaya Ziv Shani Oded Shoseyov Takahisa Hayashi 

机构地区：[1]Kyoto University, RISH, Gokasho, Uji, Kyoto 611-0011, Japan [2]Kyoto University, Institute for Chemical Research, Gokasho, Uji, Kyoto 611-0011, Japan [3]CBD Technologies, Rehovot 76100, Israel [4]Institute of Sustainability Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan

出　　处：《Molecular Plant》2009年第5期904-909,共6页分子植物（英文版）

摘　　要：In order to create trees in which cellulose, the most abundant component in biomass, can be enzymatically hydrolyzed highly for the production of bioethanol, we examined the saccharification of xylem from several transgenic poplars, each overexpressing either xyloglucanase, cellulase, xylanase, or galactanase. The level of cellulose degradation achieved by a cellulase preparation was markedly greater in the xylem overexpressing xyloglucanase and much greater in the xylems overexpressing xylanase and cellulase than in the xylem of the wild-type plant. Although a high degree of degradation occurred in all xylems at all loci, the crystalline region of the cellulose microfibrUs was highly degraded in the xylem overexpressing xyloglucanase. Since the complex between microfibrils and xyloglucans could be one region that is particularly resistant to cellulose degradation, loosening xyloglucan could facilitate the enzymatic hydrolysis of cellulose in wood.In order to create trees in which cellulose, the most abundant component in biomass, can be enzymatically hydrolyzed highly for the production of bioethanol, we examined the saccharification of xylem from several transgenic poplars, each overexpressing either xyloglucanase, cellulase, xylanase, or galactanase. The level of cellulose degradation achieved by a cellulase preparation was markedly greater in the xylem overexpressing xyloglucanase and much greater in the xylems overexpressing xylanase and cellulase than in the xylem of the wild-type plant. Although a high degree of degradation occurred in all xylems at all loci, the crystalline region of the cellulose microfibrUs was highly degraded in the xylem overexpressing xyloglucanase. Since the complex between microfibrils and xyloglucans could be one region that is particularly resistant to cellulose degradation, loosening xyloglucan could facilitate the enzymatic hydrolysis of cellulose in wood.

关 键 词：Overexpression of xyloglucanase SACCHARIFICATION transgenic poplar xylem. 

分 类 号：TS195.2[轻工技术与工程—纺织化学与染整工程] O636.1[轻工技术与工程—纺织科学与工程]