Virus-Induced Gene Silencing in the Culinary Ginger （Zingiber officinale）： An Effective Mechanism for Down-Regulating Gene Expression in Tropical Monocots  

作　　者：Tanya Renner Jennifer Bragg Heather E. Driscoll Juliana Cho Andrew O. Jackson Chelsea D. Specht 

机构地区：[1]Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA [2]Present address: Genornics and Gene Discovery, USDA, ARS, PWA, WRRC-GGD, 800 Buchanan Street, Albany, CA 94710, USA

出　　处：《Molecular Plant》2009年第5期1084-1094,共11页分子植物（英文版）

摘　　要：Virus-induced gene silencing （VIGS） has been shown to be effective for transient knockdown of gene expression in plants to analyze the effects of specific genes in development and stress-related responses. VlGS is well established for studies of model systems and crops within the Solanaceae, Brassicaceae, Leguminaceae, and Poaceae, but only recently has been applied to plants residing outside these families. Here, we have demonstrated that barley stripe mosaic virus （BSMV） can infect two species within the Zingiberaceae, and that BSMV-VIGS can be applied to specifically down-regulate phytoene desaturase in the culinary ginger Zingiber officinale. These results suggest that extension of BSMV-VlGS to monocots other than cereals has the potential for directed genetic analyses of many important temperate and tropical crop species.Virus-induced gene silencing （VIGS） has been shown to be effective for transient knockdown of gene expression in plants to analyze the effects of specific genes in development and stress-related responses. VlGS is well established for studies of model systems and crops within the Solanaceae, Brassicaceae, Leguminaceae, and Poaceae, but only recently has been applied to plants residing outside these families. Here, we have demonstrated that barley stripe mosaic virus （BSMV） can infect two species within the Zingiberaceae, and that BSMV-VIGS can be applied to specifically down-regulate phytoene desaturase in the culinary ginger Zingiber officinale. These results suggest that extension of BSMV-VlGS to monocots other than cereals has the potential for directed genetic analyses of many important temperate and tropical crop species.

关 键 词：Barley stripe mosaic virus virus-induced gene silencing VIGS Zingiber officinale Monocot. 

分 类 号：Q949.71[生物学—植物学] Q19