大鼠全脑脑片培养及缺氧缺糖模型的建立  

Whole Brain Slices Culture and Anoxia/hypoglycaemia Model Building

作　　者：杨丽君[1] 崔红[1] 杨爱君[1] 黄德庄[2] 崔雁[1] 魏田力[1] 

机构地区：[1]首都医科大学附属北京友谊医院儿科,北京100050 [2]首都医科大学附属北京佑安医院,北京100054

出　　处：《实验动物与比较医学》2009年第5期283-285,共3页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金（30872778/C1704）;北京市自然基金（NO.7072023）;首都医科大学基础、临床合作基金（NO.2006j118）

摘　　要：目的探讨离体条件下建立简单方便的新生大鼠缺氧缺糖模型的方法，为后续的研究奠定基础，从而探索可行的研究脑损伤的方法。方法在静态全脑脑片培养的基础上，采用充氮气法制备缺氧模型，将缺氧缺糖后的脑片进行冰冻切片，HE染色观察缺氧缺糖的效果，探讨该模型是否成功建立。结果全脑脑片组织生长良好；与正常对照组相比，缺氧缺糖组的脑片组织中出现核固缩及碎裂等的情况。结论成功地在离体全脑脑片培养的基础上建立了离体脑组织缺氧缺糖模型。Objective To investigate how to build up a single and convenient anoxia/hypoglycaemia model using neonatal rat brain slice culture. Methods On base of brain slice culture, nitrogen injection was used to acquire the anoxia/hypoglycaemia model. After frozen section on the brain slices, HE stain was introduced to detect the effect of anoxia/hypoglycaemia. Results Whole brain slices grew well. Karyopyknosis and nuclear fragmentation were observed in anoxia/hypoglycaemia group compared to control. Conclusions The anoxia/hypoglycaemia model was established by using brain slices in vitro.

关 键 词：离体 新生大鼠 缺氧缺糖 模型 

分 类 号：R722.6[医药卫生—儿科] R-332[医药卫生—临床医学]