RAEB型骨髓增生异常综合征患者血清蛋白质组学研究  

Serum proteomics in patients with RAEB myelodysplastic syndromes

作　　者：钟立业[1] 刘天浩[2] 李扬秋[3] 耿素霞[1] 陆泽生[1] 翁建宇[1] 吴穗晶[1] 罗成伟[1] 杜欣[1] 

机构地区：[1]广东省人民医院血液科/广东省医学科学院,广东广州510080 [2]中山大学附属第二医院肿瘤科,广东广州510120 [3]暨南大学医学院血液病研究所,广东广州510632

出　　处：《南方医科大学学报》2009年第9期1799-1801,1805,共4页Journal of Southern Medical University

基　　金："十一五"国家科技支撑计划项目(2008BAI61B02);广东科技计划基金项目(2006B36005009)

摘　　要：目的利用蛋白质组学方法,寻找与RAEB(refractory anemia with excess blasts in transformation)型骨髓增生异常综合征(MDS)相关的特异性血清蛋白质标志物。方法应用双向电泳分别分离RAEB型MDS、急性髓性白血病及正常人的血清蛋白,通过对3组图谱进行比较分析,识别差异反应的蛋白质点,根据差异反应蛋白质点位置在平行胶上找出匹配的蛋白质点,并通过基质辅助激光解吸电离飞行时间质谱对差异蛋白质点进行鉴定,通过数据库搜索鉴定出差异反应的蛋白质。结果以MDS组为参照,经过胶图分析得到7个差异蛋白质点,质谱鉴定提示4种蛋白有统计学意义,包括二肽基肽酶(dipeptidyl peptidase,DPP/CD26)、DNA聚合酶、未命名蛋白2044和类白蛋白物质。结论以2-DE为基础的血清蛋白质组学方法可用于筛选与MDS相关的蛋白质标志物;DPP在RAEB型MDS中的异常高表达提示DPP/CD26可能与进展类型的MDS相关。Objective To screen the molecular markers for refractory anemia with excess blasts in transformation （RAEB） in myelodysplastic syndromes （MDS） by serum proteome profiling. Methods The serum protein were isolated from patients with RAEB, acute myeloid leukemia or normal subjects by 2-dimensional electrophoresis （2-DE）, and the electrophoresis gels were obtained to identify the differentially reacting protein spots. The replica gels of the differentially reacting proteins were analyzed.to locate the matching protein spots, which were identified by peptide mass fingerprint based on/natrix-assisted laser desorption/ionization time of-flight mass spectrometry （MALDI-TOF-MS） and database searching. Results Seven differentially expressed proteins in RAEB were found by 2-DE. Of the 7 proteins, 4 were identified by MALDI-TOF-MS to have significantly differential expression in RAEB, including dipeptidyl peptidase （DPP/CD26）, polymerase （DNA directed） kappa, PRO2044 and an albumin-like protein. Conclusion 2-DE-fiased serum proteome profiling helps identify serum proteomic biomarkers related to MDS. DDP/CD26 has increased expression in the serum in RAEB subtype MDS, suggesting its possible role in advanced MDS.

关 键 词：骨髓增生异常综合征 血清蛋白质组学 二肽基肽酶 

分 类 号：R551.3[医药卫生—血液循环系统疾病]