通心络对家兔动脉粥样硬化模型MMP-3,9及PPAR_γ表达的影响  

Effects of Tongxinluo on MMP-3, 9 and PPAR_γ expressions in atherosclerotic rabbits

作　　者：曹鸿雁[1] 秦玲[1] 黄可欣[2] 

机构地区：[1]吉林大学第一医院心内科,吉林长春130021 [2]吉林大学基础医学院组织学与胚胎学教研室,吉林长春130021

出　　处：《南方医科大学学报》2009年第9期1887-1889,共3页Journal of Southern Medical University

基　　金：长春市科技厅资助课题(2006051);吉林省科技厅资助课题(20050408)

摘　　要：目的研究通心络对金属蛋白酶-3(MMP-3)、金属蛋白酶-9(MMP-9)和过氧化物酶体增殖激活受体γ(PPARγ)在家兔动脉粥样硬化组织中表达的影响,探讨通心络的抗AS机制。方法将实验动物家兔分为对照组(n=8)、高脂模型组(n=8)和通心络干预组(n=8);高脂模型组用高脂饲料饲养14周,通心络干预组除高脂饲养外加用通心络;应用免疫组化方法观察MMP-3、MMP-9和PPARγ在主动脉粥样硬化组织中的表达以及通心络对其表达的影响。结果高脂模型组和通心络组兔主动脉壁所含MMP-3和MMP-9的阳性标记物百分比均明显高于对照组(P<0.01),所含PPARγ的阳性标记物百分比也明显高于对照组(P<0.01),但是通心络干预组兔所含MMP-3和MMP-9的阳性标记物百分比低于高脂模型组(P<0.01),而所含PPARγ的阳性标记物百分比显著高于高脂模型组(P<0.01)。结论通心络抑制动脉粥样硬化病变部位MMP-3和MMP-9的表达,增加PPARγ的表达。其抗AS作用可能与抑制MMP-3和MMP-9及增加PPARγ的表达有关。Objective To study the effects of Tongxinluo on the expressions of matrix metalloproteinase-3 （MMP-3）, MMP-9 and peroxisome proliferators-activated receptor gamma （PPARγ） in atherosclerotic rabbits and explore the mechanism ofits anti-atherosclerotic effect. Methods Twenty-four rabbits were randomized equally into control group, atherosclerotic model group （fed with high-fat diet for 14 weeks） and＇Tongxinluo group. The expressions of MMP-3, 9 and PPARγ in the 3 groups were observed by means of immunohistochemistry. Results The expressions ofMMP-3, 9 and PPARγ in the model group and Tongxinluo group were significantly higher than those in the control group （P〈0.01）. After high-fat diet feeding for 14 weeks, Tongxinluo group showed significantly lower expressions of MMP-3 and 9 but higher expression of PPAR~ than the model group （P〈0.01）. Conclusion Tongxinluo can inhibit the expression of MMP-3 and 9 and increase the expression of PPARγ, which might be the mechanism of its anti-atberosclerotic effect.

关 键 词：通心络 氧化物酶体增殖激活受体γ 金属蛋白酶-3 金属蛋白酶-9 

分 类 号：R285.5[医药卫生—中药学]