脐血及胎盘中TXA_2、PGI_2、TXA_2R异常表达与羊水过少的相关性研究  

Correlation between oligohydramnios and abnormal expressions of TXA_2, PGI_2 and TXA_2R in the umbilical arterial blood and placenta

作　　者：王志坚[1] 余艳红[1] 陈洁[1] 赵影庭[1] 张燕[1] 

机构地区：[1]南方医科大学南方医院妇产科,广东广州510515

出　　处：《南方医科大学学报》2009年第9期1917-1918,1921,共3页Journal of Southern Medical University

基　　金：南方医科大学南方医院2007年度新业务新技术(2007008)

摘　　要：目的探讨血栓素A2(TXA2)和前列环素(PGI2)在羊水过少发病中的作用。方法用放免法检测羊水过少和正常妊娠各30例产妇脐血中TXB2和6-keto-PGF1α浓度,计算TXB2/6-keto-PGF1α比值,并用免疫组化方法检测胎盘绒毛血管内皮细胞血栓素A2受体(TXA2R)蛋白含量。结果研究组脐血TXB2浓度明显增高,与对照组相比差异有统计学意义(P<0.01);研究组6-keto-PGF1α浓度与对照组相比亦有所升高,但差异无统计学意义(P>0.05);研究组TXB2/6-keto-PGF1α明显高于对照组,差异有统计学意义(P<0.05)。羊水过少组胎盘绒毛血管内皮细胞中TXA2R阳性表达(22/30,73.3%)明显高于对照组(11/30,36.7%),两组相比差异有显著性(P<0.05)。并且羊水过少组TXA2R阳性表达以强阳性为主。结论脐血中TXA2浓度异常升高及TXA2/PGI2比例失衡与羊水过少发病有关。Objective To investigate the roles ofthromboxane A2 （TXA2） and prostaglandin I2 （PGI2） in development of oligohydmmnios. Methods The concentration of TXB2 and 6-keto-PGF1 in umbilical cord blood collected from 30 normal parturients （control） and 30 parturients with oligohydramnios was detected by radioimmunoassay to calculate the TXA2/PGI2 ratio. Immunohistochemistry was performed to detect the contents of TXA2R in vascular endothelial cell in the placental villi. Results Compared with the control group, the concentration of umbilical cord blood TXB2 in oligohydramnios group was significantly increased （P〈0.01）, but the elevation of 6-keto-PGG concentration was not statistically significant （P〉0.05）. The oligohydramnios group showed significantly higher positivity rates of TXB2 and 6-keto-PGF1 in than the control group （P〈0.01）, and the positivity rate of TXA2R in the vascular endothelial cells in the placental villi was also significantly higher in the oligohydramnios group （22/30, 77.3% vs 11/30, 36.7%, P〈0.05）. Most of the TXA2R-positive cases in the oligohydramnios group showed strong positivities of TXA2R. Conclusion Abnormal elevation of TXA2 concentration in the umbilical cord blood and the TXA2/PGI2 imbalance are responsible for the development of oligohydramnios.

关 键 词：血栓素A2 前列环素 血栓素A2受体 羊水过少 脐血 胎盘 

分 类 号：R714.2[医药卫生—妇产科学]