p38MAPK在周期性机械牵张诱导肺泡巨噬细胞表达HMGB1中的作用  

Role of p38 MAPK in cyclic mechanical stretch induced HMGB1 expression in alveolar macrophages

作　　者：丁宁[1] 肖慧[2] 高巨 许立新[1] 佘守章[1] 

机构地区：[1]广州市第一人民医院麻醉科和麻醉学实验室,广东广州510180 [2]广州市第一人民医院门诊部,广东广州510180 [3]广州中医药大学第二附属医院麻醉科,广东广州510120

出　　处：《中国病理生理杂志》2009年第10期1979-1982,共4页Chinese Journal of Pathophysiology

基　　金：广州市医药卫生科技资助项目(No.2008-YB-012);广州市医药卫生科技重点资助项目(No.2008-ZDi-14);广东省科技计划资助项目(No.2007B31509007);广东省医学科研基金资助项目(No.A2009497)

摘　　要：目的:研究p38 MAPK在周期性机械牵张诱导肺泡巨噬细胞(AM)表达高迁移率族蛋白B1(HMGB1)中的作用。方法:大鼠AM随机分为A、B、C3组,A组为对照组;B组细胞施加20%牵张应变,牵张时间为4h;C组细胞的牵张模式与B组相同,在牵张前用p38 MAPK特异性抑制剂SB203580(40μmol/L)预处理2h。利用RT-PCR法检测HMGB1 mRNA的表达,Western blotting检测HMGB1蛋白表达和p38 MAPK的活性。结果:与对照组相比,AM施加20%牵张应变可诱导HMGB1蛋白和mRNA表达明显增加、p38 MAPK活性明显增高(均P<0.05),SB203580可显著抑制牵张应变的这种诱导作用(均P<0.05)。结论:周期性机械牵张可能通过p38 MAPK信号通路,调节肺泡巨噬细胞HMGB1 mRNA和蛋白的表达。AIM： To investigate the role of p38 mitogen - activated protein kinase （MAPK） in cyclic mechanical stretch induced the expression of high mobility group box 1 protein （ HMGB1 ） in alveolar macrophages （AMs）. METHODS： AMs were cultured and seeded at 1 × 10^8 cells/L in 6 -well Bioflex cell culture plates. Subsequently, the cells were exposed to cyclic mechanical stretch at 20% （ group B） elongation using Flexercell 4000T cell stretching unit. In group C, cells were pre - treated with SB203580 （40 μmol/L） for 2 h.before mechanical stretch. Group A served as control. The expression of HMGB1 mRNA in alveolar macrophages was detected by RT - PCR. p38 MAPK activity and the expression of HMGBI protein were measured by Western blotting analysis. RESULTS： The expression of HMGBI mRNA and protein, and the activity of p38 MAPK in AMs were significantly increased in group B than those in group A （P 〈 0. 05）. SB203580, an inhibitor of p38 MAPK, significantly inhibited the inducing effect of mechanical stretch （P 〈 0. 05 ）. CONCLUSION ： Mechanical stretch regulates the expression of HMGB1 mRNA and protein in alveolar macrophages by activating p38 MAPK signal pathway.

关 键 词：机械牵张 高迁移率族蛋白质1 P38MAP激酶 肺泡巨噬细胞 

分 类 号：R322.3[医药卫生—人体解剖和组织胚胎学] R329.2[医药卫生—基础医学]