活性氧在亚砷酸钠诱导Chang肝细胞凋亡中的作用  

Reactive oxygen species play an important role in NaAsO_2-induced Chang liver cells apoptosis

作　　者：王惠惠[1] 徐苑苑[1] 郑怡[1] 张强[1] 孙贵范[1] 

机构地区：[1]中国医科大学公共卫生学院劳动卫生教研室,沈阳110001

出　　处：《中国地方病防治》2009年第5期324-326,共3页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然科学基金重点项目(No.30530640);国家自然科学基金面上项目(No.30600510);国家十一五科技支撑计划课题(2006BAI06B04)

摘　　要：目的研究在亚砷酸钠(NaAsO2)诱导人Chang肝细胞株的凋亡过程中细胞内活性氧(ROS)和还原型谷胱甘肽(GSH)的作用。方法通过流式细胞术Annexin V/PI双染法检测细胞凋亡,采用2′,7′-二乙酰二氯荧光素(DCFH-DA)检测细胞内ROS水平,应用DTNB法测定细胞内GSH含量。结果与对照组相比,在5～30μmol/LNaAsO2浓度范围内,随着NaAsO2浓度的增高,Chang肝细胞凋亡率、细胞内ROS水平及GSH含量均升高(P<0.05);5mmol/L N-乙酰半胱氨酸(NAC)可显著抑制NaAsO2诱导的细胞凋亡的发生(P<0.05)及细胞内ROS水平的升高(P<0.05),但细胞内GSH含量继续升高(P<0.05)。结论砷诱导的Chang肝细胞的凋亡可能主要取决于细胞内ROS的产生,而不是细胞内GSH含量的下降。Objective To study the role of reactive oxygen species（ROS） and reduced glutathione（GSH） in NaAsO2-induced apoptosis in Chang liver cells.Methods The apoptosis rate was assessed by AnnexinV/PI staining.The level of ROS was detected by staining cells with DCFH-DA.The level of intracellular GSH was measured with improved DTNB method.Results After treatment with 5 to 30 μmol/L NaAsO2,compared with control group,a hallmark of apoptosis and accompanied with the enhanced generation of intracellular ROS and GSH were found（P〈0.05）.The addition of N-acetyl-L-cysteine（NAC） effectively rescued the cells from NaAsO2-induced apoptosis and reduced the generation of ROS（P〈0.05）,but elevated the level of GSH（P〈0.05）.Conclusions These data suggest that the arsenic-induced Chang liver cells apoptosis is mostly dependent on generation of ROS rather than GSH depletion.

关 键 词：亚砷酸钠 N-乙酰半胱氨酸 Chang肝细胞 凋亡 活性氧 还原型谷胱甘肽 

分 类 号：R599.9[医药卫生—内科学]