小熊猫小脑皮层的组织结构及RT-97、KGF和Bax蛋白在小脑皮层的表达  

Histological Structure and Expression of RT-97,KGF and Bax Proteins in the Cerebellar Cortex of Ailurus fulgens

作　　者：高先军[1] 王昱[1] 俞诗源[1] 王悦[1] 郭婷婷[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《动物学杂志》2009年第5期32-37,共6页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30370163);甘肃省自然科学基金项目(No.ZS031-A25-005-Z);甘肃省创新人才基金项目(ZS006-016)

摘　　要：为了解小熊猫(Ailurus fulgens)小脑皮层的结构特征,观察神经丝蛋白抗体RT-97、角质细胞生长因子(KGF)及Bax蛋白在小脑皮层中的表达,利用组织学方法和免疫组织化学方法观察了小熊猫小脑皮层的显微结构,检测了RT-97、KGF和Bax蛋白的表达。结果表明,小脑皮层从外向内依次可分为分子层、Purkinje细胞层、颗粒层3层。RT-97在小熊猫小脑皮层Purkinje细胞层、颗粒层中神经细胞的轴突、分子层中颗粒细胞的轴突及小脑髓质中有阳性表达;KGF在小脑皮层分子层、Purkinje细胞层和颗粒细胞层及髓质中均有阳性表达;Bax蛋白在小脑皮层分子层、Purkinje细胞层和颗粒细胞层中有阳性表达。RT-97、KGF和Bax蛋白在小脑皮层神经结构的构筑中可能发挥着不同的功能。The histological structure of cerebellar cortex was observed and expression of RT-97, KGF, Bax proteins in the cerebellar cortex was investigated by immunohistochemistry in Ailurus fulgens. Results showed that cerebeIlar cortex of A .fulgens consists of molecular layer, Purkinje cell layer and granular layer. RT-97 appeared to express in the Purkinje cell layer, granular cell axon in granular layer and molecular layer as well as medulla. KGF- immunopositive ceils were found in all layers. Bax protein was present in the molecular layer, Purkinje cell layer and granular layer. RT-97, KGF and Bax proteins might play different roles in the construction of neuromechanism in cerebellar cortex.

关 键 词：小熊猫 小脑皮层 RT-97 KGF BAX蛋白 免疫组化 

分 类 号：Q42[生物学—神经生物学]