三角帆蚌钩介幼虫在两种寄主鱼上的寄生效果及其对寄主鱼血浆生化指标的影响  

The Host Efficiency of Two Kinds of Fish for Glochidia of Hyriopsis cumingii and Impact on Plasma Biochemical Indexes of Host Fish

作　　者：闻海波[1,2] 邱丽华[1] 顾若波[2] 徐钢春[2] 华丹 徐跑[2] 

机构地区：[1]中国水产科学研究院水产种质资源与养殖技术重点开放实验室,广州510300 [2]中国水产科学研究院淡水渔业研究中心农业部淡水鱼类遗传育种与养殖生物学重点开放实验室,无锡214081 [3]Virginia Polytechnic Institute and State University, VA USA 24061

出　　处：《动物学杂志》2009年第5期92-97,共6页Chinese Journal of Zoology

基　　金：国家基础性工作重点专项(No.2002DEB10052);中国水产科学研究院水产种质资源与养殖技术重点开放实验室开放基金项目(No.2007A003);中央级公益性科研院所基本科研业务费专项资金项目(No.6-115028)

摘　　要：利用黄颡鱼(Pelteobagrus fulvidraco)、尼罗罗非鱼(Tilapia nilotica)对三角帆蚌(Hyriopsis cumingii)钩介幼虫进行了寄生实验,分析了幼虫在寄主鱼上的寄生周期和脱落数量与寄主种类、寄主鱼单尾体重的相关关系。以未寄生幼虫的罗非鱼为空白对照,测定分析了罗非鱼在幼虫寄生前、脱落后主要血浆生化指标的变化。实验结果显示:黄颡鱼、罗非鱼平均每千克体重脱落稚贝的数量分别为(26 435±6 430)只、(26 327±3 091)只,两种寄主鱼间不存在显著差异;脱落的稚贝数量随着寄主鱼体重的增加而增加,并呈显著线性相关。在水温(24±1)℃条件下,黄颡鱼组、罗非鱼组的稚贝脱落高峰分别出现在寄生后的第8 d、第7 d,罗非鱼组表现为明显的"早脱落"现象。罗非鱼在寄生前后血浆总蛋白(TP)、白蛋白(Alb)、总糖(Glu)、总胆固醇(TC)和高密度脂蛋白(HDL)含量均未出现显著变化,而血浆甘油三酯(TG)、低密度脂蛋白(LDL)含量显著下降(P=0.001,0.020)。未寄生组罗非鱼的7项指标均未出现明显变化。实验结果将为三角帆蚌钩介幼虫变态发育相关营养因子的探讨提供理论参考。We tested the host-specificity of Triangle Muessl （Hyriopsis cumingii ）by infecting Yellow Catfish （Pelteobagrus fulvidraco ） and Nile Tilapia （Tilapia nilotica ） with its glochidia and the correlations between time course of excystment of glochidia, number of transformed juvenile and host fish species, body weight were analyzed, In addition, the plasma biochemical indexes of tilapia which were infected by glochidia of triangle mussel were determined and non-parasitized tilapia group was set up. The results showed the number of transformed juvenile from yellow catfish and tilapia per unit body weight stood at 26 435 ± 6 430, 26 327 ± 3 091 respectively, whereas there was no obvious difference. As body weight of host fish increased, the number of transformed juvenile did actually. The peak time of detached juveniles form yellow fish and tilapia appeared at the 8th, 7th day post-infection respectively with water temperature ranging from 23℃ to 25℃ . In particular, the glochidia attached tilapia detached earlier than yellow fish. There were no obvious change in concentration of plasma biochemical indexes of parasitized tilapia, such as total protein （TP）, albumin （ Alb）, glucose （ Glu）, total cholesterol （ TC ） and high density lipoprotein （ HDL）, whereas the concentrations of triglyceride （TG） and low density lipoprotein （ LDL ） significantly declined （ P = 0.001,0.020）. There was no obvious difference in concentration of all indexes of non-parasitized tilapia either. The results will provide reference to find key factors to metamorphosis development of triangle mussel.

关 键 词：三角帆蚌 钩介幼虫 寄主鱼 血浆指标 

分 类 号：S917.4[农业科学—水产科学]