液相色谱-串联质谱法测定健康人口服复方氨酚右敏溶液后的尿药浓度  

Determination of paracetamol and dextrochlorpheniramine concentrations in human urine after oral administration by LC-MS/MS

作　　者：刘丽宏[1] 潘志恒[2] 朱海静[1] 马萍[1] 李鹏飞[1] 丁春雷[1] 童卫杭[1] 

机构地区：[1]第二炮兵总医院药剂科,北京100088 [2]天津大学药物科学与技术学院,天津300072

出　　处：《中国临床药理学杂志》2009年第5期441-444,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的建立高效液相色谱-串联质谱联用法同时测定人尿中对乙酰氨基酚和右氯苯那敏(解热镇痛药)浓度及其排泄。方法12名健康志愿者按3×3拉丁方顺序进行,单剂口服给药后,按设计时间点收集尿样;尿样加入曲马多为内标,甲醇沉淀蛋白后进样,用多重反应监测,定量检测对乙酰氨基酚和右氯苯那敏的浓度。结果对乙酰氨基酚和右氯苯那敏线性范围分别为0.05～20.00μg·mL-1和0.05～20.00ng·L-1;96h内对乙酰氨基酚和右氯苯那敏低、中、高剂量组的原形药物的平均累积排泄率分别为(3.87±0.75)%、(3.86±1.33)%、(3.56±1.45)%和(8.06±3.79)%、(7.62±3.39)%、(8.10±3.42)%。结论本方法适于同时测定人尿中对乙酰氨基酚和右氯苯那敏浓度,且2者在人体内主要以非原形药排泄。Objective To develop LC-MS/MS method for the simultaneous determination of paracetamol and dextrochlorpheniramine in human plasma and to study the urinary excretions of the drugs. Method Twelve volunteers were assigned to take the drug orally on 3×3 Latin square. Paracetamol,dextrochlorpheniramine and the internal standard were extracted from plasma by methanol which used as deproteinated solvent,and detection was carried out by multiple reaction monitoring on a LC-MS/MS system. Results The assays for paracetamol and dextrochlorpheniramine were linear over the range 0.05-20.00 μg·mL^-1 and 0.05-20.00 ng·L^-1 respectively. In 96 h, the accumulated excretion rates of high, middle and low concentrations of the drugs were （ 3.87 ± 0.75） % ,（3.86 ±1.33） %, （3.56±1.45） % and （8.06±3.79 %, （7.62 ±3.39） %, （8.10 ±3.42）%, respectively. Conclusion The method established could be applied to the simultaneous determination of paracetamol and dextrochlorpheniramine in human urine. The both drugs were excreted in a form of no - prime drug in urine.

关 键 词：高效液相色谱-串联质谱联用法 对乙酰氨基酚 右氯苯那敏 尿药排泄 

分 类 号：R969.1[医药卫生—药理学] R511.7[医药卫生—药学]