红车轴草总黄酮体外抗氧化作用的研究  

Study on Antioxidation of Total Flavonoids Red Clover in Vitro

作　　者：刘宝剑[1,2] 郭延生[1] 曲亚玲[1] 刁鹏飞[1] 魏彦明[1] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]河北北方学院教务处,河北张家口075000

出　　处：《中国药学杂志》2009年第16期1234-1237,共4页Chinese Pharmaceutical Journal

基　　金：甘肃省科技支撑计划-社会发展计划资助项目(0804NKCA110);河北省科技厅科学技术研究与发展指导计划项目(07225529)

摘　　要：目的探讨红车轴草总黄酮体外抗氧化作用。方法采用分光光度法测定H2O2诱导红细胞溶血作用,用2-硫代巴比妥酸法测定小鼠红细胞膜脂质及肝组织匀浆脂质过氧化产物丙二醛(MDA)含量。结果红车轴草总黄酮在0.2～1.6mg.L-1内能抑制H2O2诱导红细胞溶血和红细胞膜脂质过氧化作用,IC50分别为0.93及0.92mg.L-1,均呈较好的量效关系;红车轴草总黄酮在4～32mg.L-1内能抑制肝组织脂质过氧化产物的形成,IC50为17.5mg.L-1。结论在一定浓度范围内红车轴草总黄酮体外具有明显的抗氧化作用。OBJECTIVE To study the anti-oxidation of the flavonoids in the red clover in vitro. METHODS Spectxophotometry was used to measure the hemolysis of the erythrocytic induced by H2O2 and TBA colorimetry to study the content of lipid peroxidation products （MDA） in the erythrocytes and the liver homogenates of mice. RESULTS The results indicated that the flavonoids of the red clover at the concentration of 0.2 1.6 mg.L^-1 significantly inhibited the erythrocytic hemolysis induced by hydrogen peroxide and the lipid peroxidation of erythrocytic membranes. 50% inhibitory concentrations （IC50） were 0.93 and 0.92 mg.L^-1 respectively, showing a appropriate concentxation-effect relationship. And they obviously prevented the formation of MDA lipid peroxidation in homogenates of mouse liver at the concentration of 4 32 mg.L^-1, with IC50 of 17.5 mg.L^-1. CONCLUSION The flavonoids of the red clover have a significant antioxidation effect within a range of certain concentration in vitro.

关 键 词：红车轴草总黄酮 抗氧化 脂质过氧化 红细胞溶血 

分 类 号：R285.5[医药卫生—中药学]