白附子提取物抑制人胃癌SGC-7901细胞增殖及诱导凋亡的机制研究  

Mechanisms of Proliferation Inhibition and Apoptosis Inducement of Rhizoma Typhonii Extracts in Human Gastric Carcinoma SGC-7901 Cells

作　　者：李艳凤[1] 唐丽萍[1] 马超[1] 马英丽[1] 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《中国药学杂志》2009年第16期1237-1240,共4页Chinese Pharmaceutical Journal

基　　金：黑龙江省自然科学基金(D200630);黑龙江省博士研究生创新科研项目(YJSCX2007-0176HLJ)

摘　　要：目的探讨白附子提取物对人胃癌SGC-7901细胞增殖抑制和凋亡诱导作用及其可能机制。方法分别采用噻唑蓝(MTT)法检测细胞增殖活性、流式细胞仪检测细胞周期及凋亡、光镜观察细胞结构变化、Hoechst33342/PI荧光染色检测细胞凋亡、Western blotting法检测caspase-3、Bax和Bcl-2蛋白表达。结果0.072～7.2mg.L-1白附子提取物处理24h后,细胞增殖明显受到抑制,且抑制率随时间延长而增加;光镜下可观察到细胞凋亡形态的改变;Western blotting结果显示,半胱氨酸蛋白酶(caspase-3)酶原蛋白的激活,Bcl-2蛋白表达的降低和Bax蛋白表达上调。结论白附子提取物通过影响SGC-7901细胞的增殖、周期和凋亡相关蛋白的表达发挥凋亡诱导作用。OBJECTIVE To explore the anti-proliferation and apoptosis-induced effects of Rhizoma Typhonii extracts on SGC-7901 cells, and possible mechanisms. METHODS SGC-7901 cell proliferative activity was measured by MTT assay. Cell cycle and apoptosis were analyzed by flow cytometry （FCM）. Cell apoptosis was observed by Hoechst33342/PI fluorescent staining. The protein levels of caspase-3, Bax and Bcl-2 were detected by Western blotting. RESULTS After exposure to Rhizoma Typhonii extracts at concentrations from 0.072 to 7.2 mg.L-1 for 24 h,cellular proliferation was inhibited with the increase of the concentration and the time. The significant apoptotic morphology changes were observed. Western blotting showed the activity of the caspase-3, increase of expression of bax protein, and decrease of expression of bcl-2 protien. CONCLUSION Rhizoma Typhonii extracts induce apoptosis by up-regulation ofbax protein expression and down-regulation of bcl-2 protein in SGC-7901 cells.

关 键 词：白附子 凋亡 半胱氨酸蛋白酶-3 B细胞淋巴瘤基因-2 BAX 

分 类 号：R965[医药卫生—药理学]