3种秦皮香豆素的毛细管区带电泳快速分析  

Rapid Determination of Fraxin,Esculin and Esculetin in Cortex Fraxini by Capillary Zone Electrophoresis

作　　者：张琦[1,2] 张新申[1] 代勇 

机构地区：[1]四川大学轻纺与食品学院,成都610065 [2]西南民族大学,成都610041 [3]成都恩威投资(集团)有限公司,成都610041

出　　处：《中国药学杂志》2009年第16期1269-1272,共4页Chinese Pharmaceutical Journal

基　　金：四川省中医药管理局重点课题(2003A36);"十一五"国家科技支撑计划(2006BAI06A18-15)子课题

摘　　要：目的考察秦皮香豆素在不同实验条件下的提取与电泳行为,建立其中主要活性成分秦皮苷、秦皮甲素、秦皮乙素的毛细管区带电泳快速定量方法。方法采用50mmol.L-1硼砂溶液为运行缓冲溶液,熔融石英毛细管有效长度45cm(75μm×53.5cm),以对羟基苯甲酸丙酯为内标,操作电压18kV,柱温25℃,于210nm波长处测定。结果在优化的电泳条件下,3种秦皮香豆素在10min内完全分离。秦皮苷、秦皮甲素、秦皮乙素的线性范围分别为:6.0～96mg.L-1(r=0.9997,n=5),7～112mg.L-1(r=0.9996,n=5)和4.7～75.2mg.L-1(r=0.9994,n=5);平均加样回收率分别为101.74%,l00.92%和102.44%,相应的RSD分别为2.18%,1.97%,2.66%(n=9)。结论本实验为秦皮苷、秦皮甲素和秦皮乙素的同时定量提供了一种快速简便的检测方法。OBJECTIVE To develop a rapid and simple capillary zone electrophoresis method for determining fraxin, esculin and esculetin in Cortex fraxini. METHODS A fused silica capillary （75 μm× 53.5 cm, with effective length of 45.0 cm） was used. The running buffer was 50 mmol·L^-1 borax solution. Propyl p-hydroxybenzoate was used as an internal standard and detection wavelength was 210nm. Applied voltage was 18kV and Capillary temperature was set at 25℃. RESULTS While all three compounds were completely separated in 10 min, the linear calibration were obtained over the range of 6.0-96 mg·L^-1 （r=0.999 7, n=5） for fraxin, 7-112 mg·L^-1 （r=0.9996, n=5） for esculin, and 4.7-75.2 mg·L^-1 （r=0.999 4, n=5） for esculetin. The average recoveries was 101.74 % （RSD=2.18%, n=9）, 100.92% （RSD=1.97%, n=9）, 102.44 %（RSD=2.66%, n=9）, respectively. CONCLUSION This method is rapid, accurate and simple for the simultaneous determination of fraxin, esculin and esculetin in Cortex Fraxini.

关 键 词：秦皮 秦皮苷 秦皮甲素 秦皮乙素 毛细管区带电泳 

分 类 号：R917[医药卫生—药物分析学]