成熟石榴叶片DNA提取方法研究  

Study on DNA Extraction Methods of Mature Pomegranate (Punica granatum Linn.) Leaves

作　　者：赵丽华[1,2] 王先磊[2] 

机构地区：[1]西昌学院园艺系,四川西昌6150013 [2]西南大学园艺园林学院,重庆市花卉生物技术研究中心,重庆400715

出　　处：《安徽农业科学》2009年第31期15141-15143,15156,共4页Journal of Anhui Agricultural Sciences

基　　金：西昌学院课题(5092);会理县科技局(XA0605)

摘　　要：[目的]为了从成熟石榴叶片中提取高质量、高产量的基因组DNA。[方法]针对成熟石榴叶片含多糖、多酚的特性,用改良CTABⅠ及改良CTABⅡ法分别提取2个品种(墨石榴和青皮软籽石榴)的成熟石榴鲜叶、冻叶、干叶基因组DNA,并通过琼脂糖凝胶电泳、紫外分光光度计、酶切鉴定。[结果]改良CTABⅡ法提取的基因组DNA电泳时点样孔干净,无拖带,OD260 nm/OD280 nm为1.7～1.9,酶切完全,其质量、产量都高于改良CTABⅠ法。[结论]改良CTABⅡ法是提取成熟石榴叶片中高质量、高产量基因组DNA的有效方法。[ Objective ] The research aimed to extract the high quality and yield genomic DNA from mature pomegranate （Punica granatum Linn. ） leaves. [ Method ] According to the character of containing amylase and polyphenol, genomic DNA in fresh leaves, frozen leaves and dry leaves of 2 kinds of mature pomegranates were extracted by improved CTAB I and CTAB I1 method. And they were identified by agarose gel electrophoresis, ultraviolet spectrophotometer method and enzyme digestion. [ Result ] The results showed that the sample hole was clean and no pulling ,OD260nm/OD280nm was 1.7 - 1.9 when the genomic DNA extracted by improved CTAB Ⅱ method was electrophoresis. And its quality and yield was high than that extracted by improved CTAB I method. [ Conclusion ] The improved CTAB Ⅱ method was the effective method of extracting the high quality and yield genomic DNA from mature pomegranate （Punica granatum Linn. ） leaves.

关 键 词：石榴 成熟叶片 CTAB法 基因组DNA 提取 

分 类 号：S665.4[农业科学—果树学]