山定子种质资源的试管苗保存  

In Vitro Conservation on Test Tube Seedlings of Malus baccata Germplasm Resources

作　　者：胡月华[1,2] 王忆[1] 许雪峰 韩振海[1] 

机构地区：[1]中国农业大学园艺植物研究所北京市果树逆境生理与分子生物学重点实验室,北京100193 [2]商丘职业技术学院,河南商丘476000

出　　处：《安徽农业科学》2009年第31期15597-15597,15619,共2页Journal of Anhui Agricultural Sciences

基　　金：北京市自然基金重点项目(6071002);科技支撑项目(2008BAD92B07)

摘　　要：[目的]建立果树砧木山定子离体保存体系。[方法]以北方常用果树砧木山定子的组培苗为试材,置于添加不同浓度甘露醇(0、5、10、15、20、25 g/L)的MS基本培养基上,分别在常温和低温(5℃)条件下进行保存,研究甘露醇浓度和温度条件对山定子试管苗保存的影响。[结果]常温下,基本培养基中添加甘露醇15 g/L的保存效果较好,试管苗可保存9个月,与对照保存8个月的效果相近;低温条件下,试管苗的保存周期明显增长,在培养基中添加10、15 g/L甘露醇的保存效果较好,保存周期延长到20个月。常温和低温下保存16个月的试管苗,经继代培养和生根培养后观察,发现其生物学性状无明显变化。[结论]常温下山定子保存最适宜的甘露醇浓度是15g/L;低温下最适宜的甘露醇浓度是10～15 g/L。[ Objective ] This study aimed to establish in vitro conservation system for fruit tree Ma!us baccata. [ Method ] With the north common fruit tree Malus baccata as the tested materials, they were cultured on the MS basic medium added by mannitol with different concn, at 0, 5, 10, 15, 20, 25 g/L for in vitro conservation at normal temperature and low temperature（5℃ ）resp. to study the effect of mannitol concn, and the temperature condition on the conservation of test tube seedlings of M. baccata. [ Result] Under normal temperature, adding the mannitol at 15 g/L into the MS medium could get better conservation and the test tube seedlings could be conserved for 9 months, with the conservation time being close to CK that had the conservation for 8 months. Under low temperature, the conservation cycle of the test tube seedlings was increased and adding the mannitol at 10 and 15 g/L into the MS medium could get better conservation, with the conservation cycle being delayed to 20 months. The observation on the test tube seedlings which were conserved for 16 months under normal temperature and low temperature and then were made for subculture and rooting culture, it was found that their biological characteristics were no obvious change. [Conclusion] The optimum mannitol concn, for M. baccata concentration was 15 g/L at the normal temperature and that was 10 - 15 g/L at the low temperature.

关 键 词：山定子 甘露醇 种质资源 离体保存 

分 类 号：S667.9[农业科学—果树学]