一种改进的构建杂交扣留cDNA文库的方法  

An Improved Method for Constructing Hybrid Arrested cDNA Library

作　　者：赵大中[1,2] 陈民[1,2] 种康 谭克辉[1,2] 

机构地区：[1]北京大学生命科学学院 [2]中国科学院植物研究所发育室

出　　处：《生物化学与生物物理进展》1998年第4期373-376,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金

摘　　要：介绍了一种改进的利用减法杂交构建λ噬菌体ｃＤＮＡ文库的方法，该方法利用处理的ｓｃＤＮＡ减去两种对照的ｍＲＮＡ富集目的ｓｓｃＤＮＡ并用磁珠法分离杂交单体，结果表明其增加了杂交单体的产量、简化了实验步骤，而且更有利于富集所需基因，是构建ｃＤＮＡ文库的一个有效改进．An improved method was utilized for constructing hybrid arrested λ phage cDNA library by subtractive hybridization, in which magnetic particles were applied to separate monomers from hybrids, and candidate sscDNAs were enriched by subtracting two control mRNAs from one treated sscDNA. The results showed that this new approach had a efficient improvement in constructing cDNA library which increased the yield of monomers, simplified experimental procedures and enriched more candidate genes.

关 键 词：CDNA文库 磁珠 减法杂交 构建 

分 类 号：Q78[生物学—分子生物学]