多重PCR检测CSF1PO,TPOX和TH01基因座在中国汉族中的多态性  

Distribution of CSF1PO,TPOX and TH01 Loci in Han Chinese

作　　者：邹浪萍 杨燕[1,2,3,4] 褚嘉佑 申滨[1,2,3,4] 李德林 

机构地区：[1]云南省高级人民法院司法鉴定科学技术研究所 [2]云南省人民医院 [3]中国医学科学院医学生物研究所 [4]昆明医学院法医学系

出　　处：《Acta Genetica Sinica》1998年第3期199-204,共6页

摘　　要：短串联重复序列（ＳＴＲ）是由几个碱基对作为核心单位串联重复形成的一类ＤＮＡ序列，应用３个ＳＴＲ基因座在同一反应体系中进行互不干扰的多重ＰＣＲ，采用高分辨力的聚丙烯酰胺凝胶电泳分离、银染法显影技术，对我国汉族的ＣＳＦ１ＰＯ，ＴＰＯＸ和ＴＨ０１等３个基因座等位基因的基因频率调查，ＣＳＦ１ＲＯ基因座，观察到９个等位基因，２２个基因型；ＴＰＯＸ基因座，观察到６个等位基因，１４个基因型：ＴＨ０１基因座，观察到６个等位基因，１９个基因型。获得了满意的结果，显示了广阔的应用前景。The application of short tandem repeat (STR) polymorphisms has stimulated progress in the elaboration of linkage maps, forensic identification and paternity determination, characterization of disease genes, and simplification and precision of DNA typing. In this report. We have carried out DNA extraction from blood multiplex amplification of CSF1PO, TPOX and TH01. By using denaturing polyacrylamide gel electrophoresis and silver stain, we investigated the allele frequencies of CSF1PO, TPOX and TH01 loci in Han group living in Yunnan provice of China. In this study, 9 alleles and 22 genotypes of CSF1PO locus were observed; In the locus TPOX,6 alleles and 14 genotypes were observed. In the TH01 locus, 6 alleles and 19 genotypes were observed.

关 键 词：STR CSF1PO TPOX TH01 中国汉族 DP PCR 

分 类 号：Q987[生物学—遗传学]