大豆灰斑病菌DNA指纹图谱初步分析  

Preliminary Analysis of DNA Fingerprinting of Cercosporidium sojinum

作　　者：刘学敏[1] 李利军 惠东威[2] 张明厚[1] 陈受宜[2] 

机构地区：[1]东北农业大学植物病理系,哈尔滨150030 [2]中国科学院遗传研究所,北京100101

出　　处：《Acta Genetica Sinica》1998年第4期362-366,共5页

基　　金：黑龙江省青年计划;中国科学院植物生物技术开放实验室基金资

摘　　要：大豆灰斑病菌具有明显的生理分化现象,鉴定病菌生理小种对于培育和推广抗病品种具有重要意义。从大豆灰斑病菌一毒力较强菌株的DNA基因组文库中筛选出2个含重复顺序的克隆,并用其对16个菌株的基因组DNA进行了Southern分析,获得了基因组特异的指纹图谱,对指纹图谱和毒力间的关系进行了初步比较。Cercosporidium sojinum of soybean possesses obvious physiological specialization, identification of physiological races is very important for breeding broad resistant cultivars. Two middle repeated sequence clones were obtained from genomic DNA library with strong virulence isolate of C. sojinum by random cloning method. We established DNA fingerprinting from 16 isolates of C. sojinum by two repeated sequence clone probes and compared relationship between DNA fingerprinting and virulence, the results showed that relationship between DNA fingerprinting and virulence appeared to be positively related.

关 键 词：大豆灰斑病 DNA指纹分析 毒力 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治] Q523[农业科学—植物保护]