嗜碱芽孢杆菌N6-27碱性纤维素酶的纯化及性质  

PURIFICATION AND PROPERTIES OF ALKALINE CELLULASE FROM ALKALOPHILIC BACILLUS N6-27

作　　者：田新玉[1] 王欣[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》1998年第4期310-312,共3页Acta Microbiologica Sinica

摘　　要：The alkaline cellulase produced by alkalophilic Bacillus sp. N6-27 was purified to electrophoresis homogeneity by (NH4)2SO4 fractionation, Sepharose CL-4B hydrophobic interaction chromatography, Bio-gel P-150 chromatography. The molecular weight and pI determined by SDS-PAGE and by PAGE-IEF were 94000 and 4.2,respectively. The optimum temperature and PH for the enzymatic catalysis were 55℃ and 8.5, respectively. The enzyme activity was stable under 50℃ and in the PH range of 6-11. The substrate was carboxymethylcellulose (CMC). The enzyme activity was strongly inhibited by Fe2 +, Cu2+ and Hg2+.The alkaline cellulase produced by alkalophilic Bacillus sp. N6-27 was purified to electrophoresis homogeneity by (NH4)2SO4 fractionation, Sepharose CL-4B hydrophobic interaction chromatography, Bio-gel P-150 chromatography. The molecular weight and pI determined by SDS-PAGE and by PAGE-IEF were 94000 and 4.2,respectively. The optimum temperature and PH for the enzymatic catalysis were 55℃ and 8.5, respectively. The enzyme activity was stable under 50℃ and in the PH range of 6-11. The substrate was carboxymethylcellulose (CMC). The enzyme activity was strongly inhibited by Fe2 +, Cu2+ and Hg2+.

关 键 词：嗜碱芽孢杆菌 碱性纤维素酶 纯化 性质 

分 类 号：Q557.103[生物学—生物化学] Q936