植物花粉管质膜类整合素蛋白的免疫印迹检测  

IMMUNOBLOTS OF INTEGRINLIKE PROTEINS IN POLLEN TUBE MEMBRANE OF HEMEROCALLIS CITRINA

作　　者：孙颖[1] 徐小冬[1] 孙大业[1] 

机构地区：[1]河北师范大学生物系

出　　处：《Acta Botanica Sinica》1998年第7期680-682,共3页Acta Botanica Sinica（植物学报：英文版）

摘　　要：植物花粉管质膜类整合素蛋白的免疫印迹检测孙颖徐小冬孙大业（河北师范大学生物系石家庄０５００１６）关键词整合素，花粉管，质膜，免疫印迹ＩＭＭＵＮＯＢＬＯＴＳＯＦＩＮＴＥＧＲＩＮＬＩＫＥＰＲＯＴＥＩＮＳＩＮＰＯＬＬＥＮＴＵＢＥＭＥＭＢＲＡＮＥＯＦＨＥＭ...Antiserum against human VnR integrin,and αv,β3 integrin subunit cytoplasmic domain in Western blots were applied to determine if integrinlike proteins be present in the pollen tube membrane of Hemerocallis citrina Baroni. The resulst showed that antiβ3 integrin subunit serum could recognize 140 kD and 97 kD bands in SDSPAGE gels under the reducing conditions,while antiserum against VnR and αv integrin could recognize 160 kD and 155 kD bands respectively under the reducing conditions,and also two small bands of higher molecular weight under the nonreducing conditions. Nonimmune serum control could not cross react with any protein bands. The present study suggest that the integrinlike protein,the receptor of vitronectin could exist in the form of αv and β3 subunits in the pollen tube membrane,with its molecular mass quite similar to that of the integrin reported in animal. 

关 键 词：整合素 花粉管 质膜 免疫印迹 

分 类 号：Q944.112[生物学—植物学]