文蛤体液蛋白体内外抗肿瘤作用的研究  

Mechanism of anticancer effect in vitro and in vivo of a novel anti-tumor protein from Meretrix Meretrix Linnaeus

作　　者：张玉艳[1,2] 张胜华[3] 宁璇璇[1,2] 王翠翠[1,2] 刘明[1] 林秀坤[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071 [2]中国科学院研究生院,北京100039 [3]中国医学科学院北京协和医学院医药生物技术研究所,北京100050

出　　处：《中国新药杂志》2009年第18期1787-1792,共6页Chinese Journal of New Drugs

基　　金：国家863计划(2007AA09Z408);国家863计划(2007AA091403)

摘　　要：目的:由文蛤体液中获得一种新的抗肿瘤蛋白。方法:采用电镜技术、流式细胞仪技术、微管蛋白α-tubulin的荧光染色技术、电泳技术,研究了MML作用后细胞凋亡、细胞周期及微管蛋白的变化情况;建立裸鼠肝癌皮下移植瘤模型,观察MML的体内抗肿瘤活性。结果:发现MML可显著抑制肿瘤细胞生长,IC50达到52.2μg·mL-1。作用机制的研究表明MML可以增加肿瘤细胞膜通透性、抑制微管蛋白聚合、使肿瘤细胞停滞在G2/M期;MML对人肝癌BEL-7402移植性肿瘤小鼠具有显著的抗肿瘤作用。结论:文蛤体液蛋白是一种新型的抗肿瘤蛋白,主要通过增加细胞膜的通透性、引起细胞凋亡、抑制微管蛋白聚合产生抗肿瘤作用。Objective：To study the mechanism of a novel antitumor protein MML, on the human hepatoma BEL-7402 cells in vitro and in vivo. Methods：Electron microscope was used to analyze the morphological change of the cancer cells, and flow cytometry was performed to study the cell cycle distribution. Tubulin immunofluorescence was carried out to investigate the effect of MML on tubulin polymerization. Results：The IC50 on BEL-7402 was about 52 μg·mL^-1. Transplanted nude mice of BEL-7402 cells were used to study the antitumor effects of MML in vivo. MML altered the tumor cell membrane permeability, induced apoptosis and inhibited tubulin polymerization. MML is a potent antitumor agent in nude mice.

关 键 词：文蛤体液 抗肿瘤蛋白 流式细胞仪 微管蛋白α-tubulin的荧光 人肝癌BEL-7402细胞 裸鼠 

分 类 号：R979.1[医药卫生—药品]