流动注射双光束光度法测定大黄中黄素含量  

Determining the Content of Flavine in Rhubarb by Flow-Injection Dual-Beam Photometry

作　　者：常慧[1] 赵珍义[2] 张娜[3] 李铁福[4] 

机构地区：[1]东莞理工学院化学与环境工程学院 [2]辽宁大学化学院,沈阳市110036 [3]辽宁中医药大学药学院,沈阳市110032 [4]沈阳药科大学制药工程学院,沈阳市110016

出　　处：《光谱实验室》2009年第5期1145-1148,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：用改进的流动注射光度法测定大黄中黄素含量。大黄素在碱性介质中生成稳定红色络合物的特性,最大吸收波长为λ=530nm,一次进样同时产生一正一负两拐点的响应曲线。利用△A(A_1—A_2)的微分光度值进行定量分析,进样频率为130次·h^(-1),线性范围为1.5-350μg·mL^(-1),相关系数r=0.9997,相对标准偏差RSD=0.97%(n=9)。方法操作简单,快速可靠,平均回收率为99.6%。A new flow-injection dual-beam photometry for the detection of flavine in rhubarb was developed based on that flavine reacts with rhubarb produces the red complex compound in alkaline medium with the maximum absorbence at 530nm,and the plus—minus inflexions happens in a sample introduction.At the sampling frequency of 130 times·h^-1,the linear range is 1.5—350μg·mL^-1,the relative coefficient is 0.9997,RSD is 0.97%（n=9）,and the average recovery is 99.6%.satisfactory results were obtained in the test.

关 键 词：双光束光度法 流动注射分析 大黄素 提取 

分 类 号：O657.32[理学—分析化学]