实验性糖尿病大鼠坐骨神经VEGF表达的动态变化  

Dynamic VEGF expression of sciatic nerve in diabetes mellitus rats

作　　者：杨薇[1] 吴江[1] 孙世博[2] 白晶[1] 

机构地区：[1]吉林大学第一医院神经内科,吉林长春130021 [2]长春市中心医院,吉林长春130051

出　　处：《中风与神经疾病杂志》2009年第5期568-570,共3页Journal of Apoplexy and Nervous Diseases

基　　金：吉林省科技厅项目(200705170)

摘　　要：目的应用免疫组织化学的方法研究实验性糖尿病(DM)大鼠坐骨神经血管内皮生长因子(Vascu-lar endothelial growth factor,VEGF)的表达变化的动态规律。方法采用大鼠腹腔内注射链脲菌素(STZ)溶液,制备实验性糖尿病大鼠模型。将80只Wistar大鼠随机分为正常对照组(NC组)、成模即刻(0w)组、成模后4w组、8w组、12w组。应用免疫组织化学的方法观察VEGF在大鼠下肢坐骨神经中的动态表达规律。结果在NC组大鼠中,坐骨神经轴索中一直没有见到VEGF棕黄色异染颗粒的表达。造模前DM各组和NC组大鼠下肢坐骨神经轴索中VEGF的表达无显著差异(P>0.05);DM组大鼠从4w开始出现VEGF在坐骨神经轴索中表达的棕黄色异染颗粒明显增多,DM4w和8w组大鼠VEGF在坐骨神经轴索中的表达,分别与NC组有极显著差异(P<0.01),但是DM4w组和8w组之间没有显著差异(P>0.05)。DM12w时轴索中的VEGF的棕黄色异染颗粒明显减少,接近正常水平,与NC组没有显著差异(P>0.05),与DM4w组和8w组分别具有极显著差异(P<0.01)。无论是NC组,还是DM各组,VEGF在坐骨神经髓鞘上始终未见阳性表达。结论正常生理情况下,VEGF在大鼠坐骨神经的轴索和髓鞘中均表达很少。高血糖状态下,VEGF在轴索的表达明显增多,髓鞘的表达没有变化。因此,VEGF主要在DM大鼠坐骨神经的轴索中表达,DM大鼠早期4w时周围神经VEGF的表达即可出现明显的增加,8w时达到高峰,此后逐渐减少,到12w时已经接近正常水平。Objective To observe the dynamic expression of vascular endothelial growth factor （VEGF） in the diabetes mellitus （DM） rats＇ sciatic nerve by immunohistochemistry methods. Methods Streptozocin （STZ） （60mg/kg body weight） intraperitoneal injection was applied to make experimental DM rat models. 80 Wistar rats were randomly divided into normal control group （NC group） ,DM 0w group,DM 4w group,DM 8w group,DM 12w group. Dynamic expressions of vascular endothelial growth factor （VEGF） were observed in the diabetes mellitus （DM） rats＇sciatic nerve by immunohistochemistry methods. Results In the NC group,VEGF expressions in the sciatic nerve axon were hardly observed. Compared with the NC group, DM group rats showed no significant differences in VEGF expressions of sciatic nerve axon before STZ injection and within 1 month after STZ injection （P 〉 0.05）. Since 1 month after STZ injection, VEGF expressions in sciatic nerve axon were increased. Compared with NC group, VEGF expressions of DM 4w and DM 8w group showed significant differences respectively（P 〈 0.01 ）and no difference between DM 4w and DM 8w group （P 〉 0.05 ）. VEGF expressions decreased distinctively and closed to normal with no difference with NC group （ P 〉 0.05） and significant difference with DM 4w and DM 8w group （P 〈0.01 ）. However,VEGF expressions of sciatic nerve myelin sheath were not observed in either NC group or DM groups. Conclusion Under normal physiological state, negative VEGF expressions were observed in either axon or myelin sheath of DM rats＇ sciatic nerve. With hyperglycemic state, VEGF expressions increased in axon but not myelin sheath. Hence, VEGF expressions were observed mostly in axons of DM rats＇ sciatic nerve. Obviously increases of VEGF expression were observed in axon of DM rats＇ sciatic nerve since 4w with a peak at 8w. Then, VEGF expression decreased gradually and approached to normal at 12w.

关 键 词：实验性糖尿病大鼠 坐骨神经 VEGF 免疫组织化学 

分 类 号：R587.2[医药卫生—内分泌] R745[医药卫生—内科学]