套式聚合酶链反应结合直接荧光法检测女性生殖道沙眼衣原体  

Chlamydia trachomatis in female genital tract detected by nested polymerase chain reaction and direct fluoscent analysis

作　　者：姚兵[1,2] 杨岳琴 朱启锭[1,2] 

机构地区：[1]解放军第四五四医院检验科 [2]南京医科大学生殖医学研究中心

出　　处：《中华医学检验杂志》1998年第6期338-341,共4页

摘　　要：目的建立一种敏感、特异、快速诊断女性生殖道沙眼衣原体（ＣＴ）感染的方法。方法应用套式聚合酶链反应（ＮＰＣＲ）技术和直接荧光（ＤＦＡ）法检测女性生殖道沙眼衣原体。对两者结果不符的标本用细胞培养法验证。结果３００份受检标本，ＤＦＡ法阳性７１份，阴性２２９份，ＮＰＣＲ法阳性９０份，阴性２１０份；其中ＮＰＣＲ和ＤＦＡ均为阳性者６８份，另有２２份ＮＰＣＲ阳性，而ＤＦＡ为阴性，再经细胞培养证实系阳性。但另有３份ＤＦＡ阳性，ＮＰＣＲ却为阴性，再经细胞培养证实亦属阳性。综合ＮＰＣＲ与ＤＦＡ并对照细胞培养，结果显示ＮＰＣＲ的敏感性为９６７％（９０／９３），特异性为１００％（２０７／２０７），ＤＦＡ的敏感性为７６３％（７１／９３），特异性为１００％（２０７／２０７）。结论ＮＰＣＲ技术与ＤＦＡ法检测ＣＴ感染，均具有高度特异性、操作简便等优势；ＮＰＣＲ的敏感性优于ＤＦＡ法；ＤＦＡ法具有费用低、重复好的特点。两法结合起来检测ＣＴ，将获得理想的检测效果。Objective To establish a method for sensitive, selective and speed diagnosis of chlamydia trachomatis (CT) infected in female genital tract. Methods CT in female genital tract was detected by NPCR and DFA. Discrepant results between NPCR and DFA were further analyzed by cell culture. Results In 300 tested samples, 68 were both positive reaction of NPCR and DFA, 22 were NPCR positive but DFA negative, whereas 3 were DFA positive and NPCR negative. These discrepant samples were further determined by cell culture and all were positive. NPCR had a sensitivity of 96% (90/93) and a specificity of 100%(207/207). DFA showed a sensitivity of 76.3%(71/93) and a specificity of 100%(207/207). Conclusions The results suggested that NPCR had a higher sensitive, selective and simple procedure, DFA is rapid, simple, sensitive and selective. Combined NPCR with DFA to detect CT, will show satisfied results.

关 键 词：衣原体感染 NPCR DFA 检测 女性 生殖系感染 

分 类 号：R711.304[医药卫生—妇产科学]