诱导子对烟草细胞辅酶Q_(10)生物合成的影响  

Effects of Elicitors on Coenzyme Q_(10) Biosynthesis in Cultured Tobacco Cells

作　　者：范海延[1] 李英华[1] 吕春茂[2] 林丽[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,沈阳市东陵路120号110161 [2]沈阳农业大学食品学院,沈阳市东陵路120号110161

出　　处：《烟草科技》2009年第10期50-53,共4页Tobacco Science & Technology

基　　金：辽宁省博士启动基金"应用烟草细胞生物反应器技术生产辅酶Q10关键技术的研究"(20081063)

摘　　要：比较了甘露醇、纤维素酶、果胶酶、水杨酸及茄子黄萎病菌和番茄叶霉病菌菌丝灭活物等几种诱导子对烟草NC89细胞生长和辅酶Q10合成的影响。结果表明:适宜浓度的甘露醇有利于NC89细胞生长,但抑制胞内辅酶Q10合成,当其浓度为5.0mg/L时,NC89细胞干重提高14.6%,辅酶Q10产量仅提高1.9%;水杨酸、果胶酶及番茄叶霉病菌诱导子对NC89细胞生长有一定抑制作用,但可促进胞内辅酶Q10的合成,当水杨酸浓度和番茄叶霉病菌诱导子浓度分别为5.0mg/L和25mL/L时,胞内辅酶Q10含量分别提高25.8%和66.1%,但辅酶Q10产量分别降低7.0%和1.9%,当果胶酶浓度为3U/L时,胞内辅酶Q10含量增加54.0%,辅酶Q10产量增加40.0%;茄子黄萎病菌诱导子及纤维素酶既有利于NC89细胞的生长,又可促进其胞内辅酶Q10的合成,当其浓度分别为25mL/L和2U/L时,辅酶Q10产量分别提高71.1%和36.6%。The effects of several elicitors, including mannitol, cellulase, pectinase, salicylic acid, eggplant verticillium wilt and tomato fulvia fulva on tobacco （NC89） cell growth and synthesis of coenzyme Q10 were compared. The results showed that proper concentration of mannitol was benefit to NC89 cell growth, but it would inhibit the intraeellular synthesis of coenzyme Q10 ; when the concentration of mannitol was 5.0 mg/L, cell＇s dry weight increased by 14.6%, while the production of coenzyme Q10 only increased by 1.9%. Salicylic acid, pectinase and tomato fulvia fulva promoted the intracellular synthesis of coenzyme Q10 though certain inhibition to tobacco cell growth. When the concentrations of salicylic acid and tomato fulvia fulva were 5.0 mg/L and 25 mL/L respectively, the content of intracellular coenzyme Q10 increased by 25.8% and 66.1% , but the production of coenzyme Q10 decreased by 7.0% and 1.9% respectively. The content and production of coenzyme Q10 could be improved by 54.0% and 40.0% respectively with the concentration of pectinase at 3 U/L. Eggplant verticillium wilt elicitor and eellulase were beneficial to both cell growth and synthesis of intracellular coenzyme Q10, when their concentrations were 25 mL/L and 2 U/L, the production of coenzyme Q10 increased by 71.1% and 36.6% , respectively.

关 键 词：辅酶Q10 诱导子 烟草 NC89 细胞 

分 类 号：S572.01[农业科学—烟草工业]