黄连解毒汤对动脉粥样硬化大鼠血sICAM-1和sVCAM-1的影响  

The effects of huang lian jie du decoction on immune-inflammatory cytokine sICAM-1 and sVCAM -1 in rats with atherosclerosis

作　　者：李国强[1] 黄萍[2] 程穗茗[1] 罗承锋[1] 刘世明[1] 郭重仪[2] 操红缨[2] 

机构地区：[1]广州医学院第二附属医院心内科,广州心血管疾病研究所,广州510260 [2]广州中医药大学中医学院,广州510405

出　　处：《广东医学》2009年第11期1604-1606,共3页Guangdong Medical Journal

基　　金：广东省中医药管理局立项项目(编号:2007139)

摘　　要：目的探讨黄连解毒汤对动脉粥样硬化大鼠血清中细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)水平的影响及其作用机制。方法雄性SD大鼠,随机分成正常对照组、模型对照组、黄连解毒汤低中高3个剂量组及阿托伐他汀组,每组10只。除正常对照组喂普通饲料外,其余喂以高脂饲料,同时定期灌服维生素D3(60万U/次,第2,4,6周)造模8周,从第6周开始每天灌胃给药1次,黄连解毒汤低剂量组2.7g/kg,中剂量组5.3g/kg,高剂量组10.6g/kg,阿托伐他汀钙片组1.8mg/kg,正常对照组灌服等量蒸馏水,连续4周。结果黄连解毒汤中剂量组,高剂量组及阿托伐他汀组均与模型对照组的ICAM-1,VCAM-1表达均比模型对照组低(P<0.05～0.01)。结论黄连解毒汤对动脉粥样硬化起到一定干预作用,其机制可能与抑制炎症反应有关。Objective To study the effect of huang ]Jan jie du decoction on immune - inflammatory cytokine sICAM - 1 and sVCAM - 1 in rat serum and its molecular mechanism. Methods Male rats were divided into 6 groups, 5 groups were fed with cholesterol diet for 10 weeks and the other group was fed with normal diet. At the sixth week 3 groups were treated with low dose, moderate dose and the high dose of huang lian jie du decoction, one group with atorvastatin for 4 weeks （ except the nomal group ） Levels of slCAM - 1 and sVCAM - 1 were measured with ELISA after 10 weeks. Results Levels of slCAM - 1 and sVCAM - 1 in the model group were significantly higher than that of normal group. Compared with the model control group,levels of slCAM - 1 and sVCAM - 1 in moderate dose huang lian jie du decoction group and high dose group were significantly lower than that of the model control group. Conchlsion This study shows huang lian jie du decoction influences levels of slCAM -1 and sVCAM -1 and it may be helpful in the treatment of atherosclerosis.

关 键 词：动脉粥样硬化 动物实验 黄连解毒汤 ICAM-1 VCAM-1 

分 类 号：R318.01[医药卫生—生物医学工程] R587.2[医药卫生—基础医学]